NOL8 Protein (AA 1-1147) (Strep Tag)
Crystallography grade
NOL8
Origin: Mouse
Host: Tobacco (Nicotiana tabacum)
Recombinant
≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, SDS, WB
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- Target See all NOL8 products
- NOL8 (Nucleolar Protein 8 (NOL8))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1147
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This NOL8 protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MQGNREMKRL FVGGLGQGIS ETDLQNQFGR FGEVSDVEII TRKDDQGNSQ KVFAYVNIQI TEADLKKCMS ILNKTKWKGG TLQIQLAKES FLHRLAQERE DAKAKKEKST TGNPTLLEKM GAVDFHMKAV PGTEVPGHKN WVVSKFGRVL PVLHLKNQQK HKIMKYDPSK YCHNIKKIPE NLTETTPIAE LTWELEGGND PMSKKRRGEF SDFHIPPQKV KKVQKSNDPM ESKVSNIGLR TNQVMEKNKS THPVTAHGTA PSTVNPSKQL LVSSSGTQKP KHVVFHNSDF EIIWNKSSMS DDDVDSEDEL KMMIAKEENR EKPGHSSVNE SEHDTFEVVR DDFKSNIHKL SSSVSLGNNH EYDSSDTDEI IAMKTKNAKV KNSAESSQPE RTVSKKSSFQ KIEPSNDCIK VQGINSNKES ALCHGVKFVN PKFPPDSSGS DSEESEEDEE YKVLMENCPR VSLTLADLEQ LAGSHRKFPG KDSETNGPQN DSHCKFDTTS KNPKTSGDLY NGRQQCILPE EIVASLLEDE NTYSKQKSEE DILKPKFQAF KGIGCLYAKE SVDKTLKENI AFNTGGGHHS SLKHEDHNRS LMENGSKCVN GSSSKLTSCQ PAKKVNDPNH IQPPKRQCTF ENQNHKVMSS TSCDKGSTNP LPGPLPLKAK TSLHLSANSH KVDSDGDACH WPESRKALEK ERTNLSNLES LEKSSKVSPR EDPQKSPAGF SLSDSNASCI NAKDKQAEDN QKRLAALAAW QKAREVQKKL VHSALANLDG HPEDKKTHIV FASDNESETE ETSTQEQSCP EKELMKESVS KSPGKLFDSS DDEDSDSKED STRFSIKPQF EGRAGQKLMD LQSQFGSDER FRMDSRFLES DSEDEKKELN EDKVNEDELA AEKKKTLNVV QSVLNINVNN PTNKGSVAAK KFKDIVHYDP TKHDHAIYER KQEDKEKESK ATRKKKKEEA EKLPEVSQDM YYNIAADLKE IFQSMSNTDE KEEDVPRTEA GAREGTGKIR NAETLACEPE QTTGFTFSFF DSATKDEKDA TYRIELVKHG KIVCPNDPRF QDSSSEEEDI AEEADHSKPS PGEAVPENEA IRFFFFSEND DRLRGSNLFW SGMGGSISRN SWEARTSSLL LECRKKHKEA KRKVKAN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- NOL8 (Nucleolar Protein 8 (NOL8))
- Alternative Name
- Nol8 (NOL8 Products)
- Synonyms
- C9orf34 Protein, NOP132 Protein, bA62C3.3 Protein, bA62C3.4 Protein, 4921532D18Rik Protein, 5730412B09Rik Protein, si:ch211-103f16.2 Protein, DKFZp459O1154 Protein, nucleolar protein 8 Protein, NOL8 Protein, Nol8 Protein, nol8 Protein
- Background
- Nucleolar protein 8,FUNCTION: Plays an essential role in the survival of diffuse-type gastric cancer cells. Acts as a nucleolar anchoring protein for DDX47. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells (By similarity). {ECO:0000250}.
- Molecular Weight
- 128.6 kDa
- UniProt
- Q3UHX0
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