TEAD4 Protein (AA 2-427) (His tag)

Catalog No. ABIN3135537

Product Details

Target: TEAD4 (TEA Domain Family Member 4 (TEAD4))

Protein Type: Recombinant

Protein Characteristics: AA 2-427

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This TEAD4 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: TSNEWSSPDS PEGSSISGGS QALDKPIDND AEGVWSPEIE RSFQEALAIY PPCGRRKIIL TEEGKMYGRN ELIARHIKLR TGKTRTRKQV SSHIQVLARR KAREIQAKLK DQAAKNKALQ SMAAMSSAQI VSATAFHSKM ALARGPGYPA ISGFWQGALP GQPGTSHDVK PFSQNTYPVQ PPLPLPGFES PAGPTPSPSA PLAPPWQGRS IASSKLWMLE FSAFLERQQD PDTYNKHLFV HISQSSPSYS DPYLETVDIR QIYDKFPEKK GGLKELFERG PSNAFFLVKF WADLNTNIDD EGSAFYGVSS QYESPENMII TCSTKVCSFG KQVVEKVETE YARYENGHYL YRIHRSPLCE YMINFIHKLK HLPEKYMMNS VLENFTILQV VTNRDTQETL LCIAYVFEVS ASEHGAQHHI YRLVKE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Tead4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TEAD4 (TEA Domain Family Member 4 (TEAD4))

Alternative Name: Tead4 (TEAD4 Products)

Synonyms: TEAD4 Protein, rtef1 Protein, tef-3 Protein, eftr-2 Protein, tefr-1 Protein, tcf13l1 Protein, hrtef-1b Protein, xdtef-1 Protein, xlDTEF-1 Protein, RTEF-1 Protein, TEF-1 Protein, TEF-3 Protein, EFTR-2 Protein, RTEF1 Protein, TCF13L1 Protein, TEF3 Protein, TEFR-1 Protein, hRTEF-1B Protein, ETFR-2 Protein, Etfr2 Protein, FR-19 Protein, TEAD-4 Protein, Tcf13r1 Protein, Tef3 Protein, Tefr Protein, Tefr1 Protein, Tefr1a Protein, TEA domain transcription factor 4 Protein, TEA domain transcription factor 4 S homeolog Protein, TEA domain family member 4 Protein, TEAD4 Protein, tead4 Protein, tead4.S Protein, Tead4 Protein

Background: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif (By similarity). Might play a role in the embryonic development of skeletal muscle. {ECO:0000250}.

Molecular Weight: 48.9 kDa Including tag.

UniProt: Q62296

Pathways: Regulation of Lipid Metabolism by PPARalpha