PLEKHG2 Protein (AA 1-1340) (Strep Tag)

Catalog No. ABIN3135706

Product Details

Target: PLEKHG2 (Pleckstrin Homology Domain Containing, Family G (With RhoGef Domain) Member 2 (PLEKHG2))

Protein Type: Recombinant

Protein Characteristics: AA 1-1340

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PLEKHG2 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MPEGARGLSL PKPSLRLGCG HQGEVCDCAA VSDTPTAQAA TTMASPRGSG SSTSLSTVGS EGDPSPACSA SRPEPLPEPP IRLHLLPVGI QGSVKPSRLE RVAREIVETE RAYVRDLRSI VEDYLGPLMD GRALGLNMEQ VGTLFANIED IYEFSSELLE DLEGCSSAGG IAECFVQRSE DFDIYTLYCM NYPSSLALLR ELSVSPPATL WLQERQAQLR HSLPLQSFLL KPVQRILKYH LLLQELGKHW AEGPDSGGRE MVEEAIVSMT AVAWYINDMK RKQEHAARLQ EVQRRLGGWT GPELSAFGEL VLEGTFRGGG GGGPRLRGGE RLLFLFSRML LVAKRRGPEY TYKGHIFCCN LSVSETPRDP LGFKVSDLTI PKHRHLFQAK NQEEKRLWIH CLQRLFFENH PASIPAKAKQ VLLENSLHCA PKSKHIPEPP TSPLDSPRPR DAPGFTPGRR NPAPSPRLSG SRRGRRQSEP AKEAYVIFPQ NDKPQVKHAG SEGELHPSSE LQPVSASGLP EDLEDAGPPT LDPSGTSITE EILELLNQRG LRDSGPATHD IPKFPRDSRV PVESEPLPFQ SLPSRESSEE EEEEDLETDE REPSPLHVLE GLEGSSAAEI PCIPSLTDIP SEVPSLPEIP EAPCLPCLSD ISGVFEVPCL SPTSTVPDIP SLATTPSFPC GSWLPGPLQE AAQPQATRRE LLSGSNPGRL SESPSESREG QEDDTEGVSF SAVQREAGTS VQGFPEELEY RSCSEIRSAW QALEQGQLAR PGFPEPLLIL EDSDLRGGST SGKTGMPHSE RSASRVRELA RLYSERIQQM QRAETRASTN APRRRPRVLA QPQPSPCPPQ EEAEPGALPA FGHVLVCELA FPLNCTQESV PLGPAVLVQA ATPLCIQGDD LSGQNLNVSD LSKQGHLSSN SIPPPVPLPG QSNFQNIQVP STSLLPKQEP PDVQVPTAST LPDTSQLQSQ VPAATPSAGH RNCVEIQVQS TTSLPGQECQ ADTVALSKQE GHEDSQNPNK APGAEQRDVS IDQGLAVVGG RPVSPLPVCT SSPDQQIPAT TPLPLSTDFP DMEGPGALPL PTQEGRPDCS IPCNPLPSLS QDVQVPAVIP VSQLQGLTDT RATVPLSSHK QEDAPECLGP EPSLTDTPAP RLLSSLGQQN TTDGPVSAAA VPLTEQGCSQ DLQGLITSPV QTTMELPKPR GLVSRVATSE SLDLTPPHSP SLSTRQLLGP SAAALSRYLA ASYISQSLAR RQGPGGEGTV ASQGHWSSSA PTSRAPSPPP QPQPPAPPAR RLSYATTVSI QVGGGGRLRP AKAQVRLNHP ALLAAPHPGA VGPSQGPGGS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PLEKHG2 (Pleckstrin Homology Domain Containing, Family G (With RhoGef Domain) Member 2 (PLEKHG2))

Alternative Name: Plekhg2 (PLEKHG2 Products)

Synonyms: ARHGEF42 Protein, CLG Protein, AI194308 Protein, Clg Protein, Cslg Protein, pleckstrin homology and RhoGEF domain containing G2 Protein, pleckstrin homology domain containing, family G (with RhoGef domain) member 2 Protein, PLEKHG2 Protein, Plekhg2 Protein

Background: Pleckstrin homology domain-containing family G member 2 (PH domain-containing family G member 2) (Common site lymphoma/leukemia guanine nucleotide exchange factor) (Common site lymphoma/leukemia GEF),FUNCTION: May be a transforming oncogene with exchange activity for CDC42. May be a guanine-nucleotide exchange factor (GEF) for RAC1 and CDC42 (PubMed:11839748). Activated by the binding to subunits beta and gamma of the heterotrimeric guanine nucleotide-binding protein (G protein) (By similarity). Involved in the regulation of actin polymerization (By similarity). {ECO:0000250|UniProtKB:Q9H7P9, ECO:0000269|PubMed:11839748}.

Molecular Weight: 143.4 kDa

UniProt: Q6KAU7