AHDC1 Protein (AA 1-1594) (Strep Tag)

Catalog No. ABIN3135775

Product Details

Target: AHDC1 (AT Hook, DNA Binding Motif, Containing 1 (AHDC1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1594

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This AHDC1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MRVKPQGLVV TSSAVCSSPD YLREPKYYPG GPPTPRPLLP TRPPASPPDK AFSTHTFSEN PRPPPRRDPS SRRPPVLAKG DDLLPPRAAR PVSQAHCPSP APDNSSLRHW DNGRVNLRPV VQLIDIMKDL TRLSQDLQHS GVHLDCGGLR LSRPPAPPPG DLQYSFFSSP SLANSIRSPE ERANPHTKSE RPSHPLYEPE PEPRDSPQPG QGHGPGAAAT ATGLPPEPEP DGPDYSELAD ADILSELASL TCPEAQLLEA QALEPPSPQP EPQLLDPQPR FLDPQALEPL GEGLELPPLQ PLADPLGLPS LTLQALDTLP DSLESQLLDP QALDPLPKLL DVPGRRLEPQ QSLGHCQLAE PLRLDLCSPH GPPGPEGHPK YALRRTDRPK ILCRRRKAGR GRKADSGPEG RLLPLPMPTG LAAALAEPPP LPPPPPPTLS GPGPVPELEP ESSQTPMVPT RKGKCRGVRR MVVKMAKIPV SLGRRNKTTY KVSSLSSSLS VEGKELGLRV SSEPTPLLKM KNNGRNVVVV FPPGEMPIIL KRKRGRPPKN LLLGPGKPKE PTVVAAEAAT VTAATMAMPE VKKRRRRKQK LASPQPSYAA DANDSKAEYS DVLAKLAFLN RQSQCAGRCS PPRCWTPSEP ESVHQAPDTQ SISQFLHRVQ GFRRRGGKTG GFGGRGGGHA AKAARCSFSD FFEGIGKKKK VVAVAAPGLV GPGLTELGHP RKRGRGEVDA VTGKPKRKRR SRKNGTLFPE QVPSGPGFGE AGAEWVGDKG GGWAPHHGHP GGQAGRNCGF QGTEARAFAS TGLESGASGR GSYYAGAPSG QTELSQERQN LFTGYFRSLL DSDDSSDLLD FALSASRPES RKASGTYAGP PSSALPAQRG LATFPSRGAK ASPVAVGSSG AGADPSFQPV LPSRQTFPPG RATSYGITPA TSDCRAAETF PKLAPPPSAV ARSPTTHPPA NTYPPQYGGY GAGQSVFASA KPFSGQDCAN SKDCSFAYGS GNSLPASPSS AHSAGYAPPP TGGPCLPPSK ASFFNSSEGG PFSGSAPTPL RCDSRASTVS PGGYMVPKGT TASAASVASS SSSSFQPSPE NCRQFVGASQ WPFRQGYGGL DWASEAFSQL YNPNFDCHGS EPNVILDISN YTPQKVKQQT AVSETFSESS SDSTQFSQPV GGGGFRRANS EASSSEGQSS LSSLEKLMMD WNEASSAPGY NWNQSVLFQS SSKPGRGRRK KVDLFEASHL GFSTSTSATA SGYPSKRSTG PRQPRGGRGS GACSAKKERG GTAAKAKFIP KPQPVNPLFQ DSPDLGLDYY SGDSSMSPLP SQSRAFGVGE RDPCDFMGPY SMNPSTPSDG TFGQGFHCDS PSLGAAELDG KHFPPLAHPP TVFDAGLQKA YSPTCSPTLG FKEELRPPPS KLTACEPLKH GLQGASLSHA AQAHLSCRDL PLGQPHYDSP SCKGTAYWYP PGSAARSPPY EGKVGSGLLA DFLGRTEAVC LSAPHLASPP ATPKADKEPL EMARPPGPPR GPAAATAGYG CPLLSDLTLS PVPRDSLLPL QDTAYRYPGF MPQAHPGLGG GPKSGFLGPM AEPHPEDTFT VTSL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: AHDC1 (AT Hook, DNA Binding Motif, Containing 1 (AHDC1))

Alternative Name: Ahdc1 (AHDC1 Products)

Synonyms: CL23945 Protein, DJ159A19.3 Protein, RP1-159A19.1 Protein, D030015G18Rik Protein, AT-hook DNA binding motif containing 1 Protein, AT hook, DNA binding motif, containing 1 Protein, AHDC1 Protein, Ahdc1 Protein

Background: Transcription factor Gibbin (AT-hook DNA-binding motif-containing protein 1),FUNCTION: Transcription factor required for the proper patterning of the epidermis, which plays a key role in early epithelial morphogenesis (PubMed:35585237). Directly binds promoter and enhancer regions and acts by maintaining local enhancer-promoter chromatin architecture (By similarity). Interacts with many sequence-specific zinc-finger transcription factors and methyl-CpG-binding proteins to regulate the expression of mesoderm genes that wire surface ectoderm stratification (By similarity). {ECO:0000250|UniProtKB:Q5TGY3, ECO:0000269|PubMed:35585237}.

Molecular Weight: 168.1 kDa

UniProt: Q6PAL7