ST18 Protein (AA 1-1045) (Strep Tag)

Catalog No. ABIN3136003

Product Details

Target: ST18 (ZNF387) (Zinc Finger Protein 387 (ZNF387))

Protein Type: Recombinant

Protein Characteristics: AA 1-1045

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This ST18 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MDAEVEDKTL HTLSKGTEVP MDSLIPELRV PYDCSMAKKR RAEEQASGVP INKRKSLLMK PRHYSPDMGC KESPDNRNED DGLLETNDHA TADEIMVKSM DETLHLPAQD SSLQKKDQYT CYPELMVKSL VHLGKFEESE SVQTTCENLN GSSIQSLKAE SDEAHEGSMV HSDNGRDKVH HSQPPFCSSG DSESDSDSAE NGWGNGSNSS EDTDTHKGPK HKLTYNRKDL LEVPEIKAED DKFIPCENRC DSDTDGRDPQ NSHMEPLVVK AQPSFPEVEE GESLATVTEE PAEVEKAKGN LSLLEQAIAL QAERGSVFHH TYKELDRFFL DHLARERRQP RVTDANGRQI FTNKHSPRPE RREAKCPIPG CDGTGHVTGL YPHHRSLSGC PHKVRVPLEI LAMHENVLKC PTPGCTGRGH VNSNRNTHRS LSGCPIAAAE KLAMTQDKSQ LDSSQTGQCP EQAHRVNLVK QIEFNFRSHA ITSPRASASK EQEKFGKVPF DYASFDAQVF GKRPLLQTGQ GQKAPPFPES KHFSNPVKFP NGLPSAGAHT QSTVRASSYG HGQYSEDTHI AAAAAILNLS TRCREATDIL SNKPQSLRAK GAEIEVDENG TLDLSMKKNR IHDKSIPPTS SPTTITTPSS SPFNASSLLV NAAFYQALSD QEGWNVPINY SKSHGKTEEE KEKDPVNFLE NLEEKKFAGE ASIPSPKPKL HTRDLKKELI TCPTPGCDGS GHVTGNYASH RSVSGCPLAD KTLKSLMAAN SQELKCPTPG CDGSGHVTGN YASHRSLSGC PRARKGGIKM TPTKEEKEDS ELRCPVIGCD GQGHISGKYT SHRTASGCPL AAKRQKENPL NGAPLSWKLN KQELPHCPLP GCNGLGHVNN VFVTHRSLSG CPLNAQAIKK VKVSEELMTI KLKATGGIDG DEEIRHLDEE IKELNESNLK IEADMMKLQT QITSMESNLK TIEEENKLIE QSNESLLKEL AGLSQALISS LADIQLPQMG PINEQNFEAY VNTLTDMYSN LEQDYSPECK ALLESIKQAV KGIHV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ST18 (ZNF387) (Zinc Finger Protein 387 (ZNF387))

Alternative Name: St18 (ZNF387 Products)

Synonyms: AV348974 Protein, Myt3 Protein, NZF-3 Protein, Nzf3 Protein, mKIAA0535 Protein, r-MyT3 Protein, ZC2HC10 Protein, ZNF387 Protein, suppression of tumorigenicity 18 Protein, ST18, C2H2C-type zinc finger Protein, St18 Protein, ST18 Protein

Background: Suppression of tumorigenicity 18 protein,FUNCTION: Repressor that binds to DNA sequences containing a bipartite element consisting of a direct repeat of the sequence 5'-AAAGTTT-3' separated by 2-9 nucleotides. Represses basal transcription activity from target promoters (By similarity). {ECO:0000250}.

Molecular Weight: 114.8 kDa

UniProt: Q80TY4