CLEC16A Protein (AA 1-1036) (Strep Tag)

Catalog No. ABIN3136010

Product Details

Target: CLEC16A (C-Type Lectin Domain Family 16, Member A (CLEC16A))

Protein Type: Recombinant

Protein Characteristics: AA 1-1036

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This CLEC16A protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MFGRSRSWVG GGHSKSSRNI HSLDHLKYLY HVLTKNTTVT EQNRNLLVET IRSITEILIW GDQNDSSVFD FFLEKNMFVF FLNILRQKSG RYVCVQLLQT LNILFENISH ETSLYYLLSN NYVNSIIVHK FDFSDEEIMA YYISFLKTLS LKLNNHTVHF FYNEHTNDFA LYTEAIKFFN HPESMVRIAV RTITLNVYKV SLDNQAMLHY IRDKTAVPYF SNLVWFIGSH VIELDNCVQT DEEHRNRGKL SDLVAEHLDH LHYLNDILII NCEFLNDVLT DHLLNRLFLP LYVYSLENPD KGGERPKISL PVSLYLLSQV FLIIHHAPLV NSLAEVILNG DLSETYTKPA QDVPRSSAKP SIRCFIKPTE TLERSLEMNK HKGKKRMQKR PNYKNVGEEE DEERGSAEDA QEDAEKTKEI EMVIMKLGKL SEVAAAGTSV QEQNTTDEEK SAATNSENAQ WSRPFLDMVY HALDSPDDDY HALFVLCLLY AMSHNKGMDP EKLKRIQLPV PSEAEKTTYN HLLAERLIRI MNNAAQPDGR IRLATLELSC LLLKQQVLTS SGCVIKDVHL ACLEGAREES VHLVRHFYKG EEIFLDMFED EYRSMTIKPM NVEYLMMDAS ILLPPTGTPL TGIDFVKRLP CGDVEKTRRA IRVFFMLRSL SLQLRGEPET QLPLTREEDL IKTDDVLDLN NSDLIACTVI TKDGGMVQRF LAVDIYQMSL VEPDVSRLGW GVVKFAGLLQ DMQVTGVEDD SRALNITIHK PASSPHSKPF PILQATFVFS DHIRCIIAKQ RLAKGRIQAR RMKMQRIAAL LDLPIQPTTE VLGFGLCSSS SSSQHLPFRF YEQCRRGSSD PTVQRSVFAS VDKVPGFAVA QCINQHSSPS LSSPSPPFAS GSPGGSGSTS HCDSGGSSSA PSATQSPADA PTTPEQPQPH LDQSVIGNEM DVNSKPSKNS SARSSEGETM HLSPSLLPAQ QPTISLLYED TADTLSVESL TIVPPVDPHS LRALSGISQL PTLPAADTET PAEGAVNPEP AEPTEH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CLEC16A (C-Type Lectin Domain Family 16, Member A (CLEC16A))

Alternative Name: Clec16a (CLEC16A Products)

Synonyms: CLEC16A Protein, Gop-1 Protein, KIAA0350 Protein, 4932416N17Rik Protein, curt Protein, mKIAA0350 Protein, RGD1305773 Protein, C-type lectin domain family 16 member A Protein, C-type lectin domain containing 16A Protein, C-type lectin domain family 16, member A Protein, CLEC16A Protein, clec16a Protein, Clec16a Protein

Background: Protein CLEC16A,FUNCTION: Regulator of mitophagy through the upstream regulation of the RNF41/NRDP1-PRKN pathway. Mitophagy is a selective form of autophagy necessary for mitochondrial quality control. The RNF41/NRDP1-PRKN pathway regulates autophagosome-lysosome fusion during late mitophagy. May protect RNF41/NRDP1 from proteasomal degradation, RNF41/NRDP1 which regulates proteasomal degradation of PRKN. Plays a key role in beta cells functions by regulating mitophagy/autophagy and mitochondrial health. {ECO:0000269|PubMed:24949970}.

Molecular Weight: 116.2 kDa

UniProt: Q80U30