KIAA0284 Protein (AA 1-1574) (Strep Tag)

Catalog No. ABIN3136012

Product Details

Target: KIAA0284 (CEP170B) (Centrosomal Protein 170B (CEP170B))

Protein Type: Recombinant

Protein Characteristics: AA 1-1574

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This KIAA0284 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MSVTSWFLVS SSGTRHRLPR ELIFVGRDEC ELMLQSRSVD KQHAVINYDQ DRDEHWVKDL GSLNGTFVND VRIPDQKYIT LKLNDVIRFG YDSNMYVLER VQHRVPEEAL KHEKYTSQLQ VSVKVSAPKR GDALPDHTPY CESSQPRPEK GDRRHGAEAV AYRTPLYGQP SWWGEDDSGA PSEDRHQEEP YSERPKDLAQ QNGELDSCRA PAEPPDYSFR REPSYFEIPT KETPQPPRLP EVPTQEVPTK DQEAGVGGTA PVVQSHASFT IEFDDCSPGK VKIKDHITKF SLRQRRAPSK ETTPVETVSA ETKVADWLVQ NDPSLLRRDG PGDDRHSTKS DLPVHTRTLK GHKHEDGTQS DSEDPLAKTA SVSGASAEAS GEQVRLQRQI KRDPQELLHN QQAFVIEFFD GDTPRKKRSQ SFTHTPPADP KADKRRGPGT SDRDRPGVSV RATGSSSGPQ RASSLKREKT EERLGNTSPV PRASTRSFGS VGRRSRLAQD FMAQCMRDSS PATRPAPEKT PPVLPAPLTP RGASPVTPST TPPPPTDPQL TKARKQEEDD SLSDAGTYTI ETEAQDQEVE EARRMIDQVF GVFESPELSR VSSATFRPVI RGDKDESSDG GMAQRMALLQ EFASRAPGMA PQMEQQSLLV PGSPGGQKWV SRWASLADSY SDAGLPEDGP GRRTGEPEGP LPVRTRRLLP QLPSGRADSP AGLEAARRNG PGPPELGSEP ANCLIGQEDL DPDSLSDASG SDGGRGPEPG TERQEDLAWV RGRRSPRAPG ELVPTSFFIG DQNGEATFPK KSFVGPGEVD GPGRVVQTSP SARDGLYVSS NGRMVIQLRS GRSPEPDPAP PKETLTFARQ ESFTKEPTSG PPAPGKLPHI SSHPLLQDLA AARASRLDFH AQDTHLILKE TETALAALEA RLRSKSADEC DGGSTPRPPE DSLSGDSDVD TASTISLLSG KNGPSPTTPQ TPGPQKESPL SPPTVPDPGG ATPGSARERM SERQHRPTPA DLGPGDTSRR AAMRRGHGSR GSLDWPEEER GSGLAHLPSS NHETPEATLA GRQGPRRKPA APPPSPAARE EQSRSSATAQ KVQQALTRSN SLSTPRPTRA SRLRRARLGD ASDTEAVDGE RGTAANPEPA NRAAPEQAKK LTRLDILAMP RKRAGSFTGP SDSETAPART GFSGRSAELY STSRKPTIAE ARAAAKKAAA TAANTGPRQP FSRARPGSAR YSSNTRRRQQ GSDYTSTSEE EYGSHHSSPK HTRSHASTAT QTPRGSSSTR ARSQGPRDTD DDEEEPDPYG FIVQTAEIAE IARLSQTLVK DVAILAREIH DVAGDGDSLG SPGPTRSPSL GNVPNTPAST ISAREELVQR IPEASLNFQK VPPGSMNSHN LDQNMNDSRD DALTNKTRPR NREEVIFDNL MLNPVSQLSH AIRENTEHLA EKMKVLFQNT GRAWEDLEAR INSENEVPIL KTSNKEISSI LKELRRVQKQ LEVINAIVDP SLNLDLLMGN RAPSGSGQPG LGKARPAAQS STSPASVDTL LPALPLRSFP QRANCGPPGL PEPAFLPDAE RFLI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: KIAA0284 (CEP170B) (Centrosomal Protein 170B (CEP170B))

Alternative Name: Cep170b (CEP170B Products)

Synonyms: kiaa0284 Protein, CEP170R Protein, FAM68C Protein, KIAA0284 Protein, AI466840 Protein, AW555464 Protein, Kiaa0284 Protein, mKIAA0284 Protein, centrosomal protein 170B S homeolog Protein, centrosomal protein 170B Protein, cep170b.S Protein, CEP170B Protein, Cep170b Protein

Background: Centrosomal protein of 170 kDa protein B (Centrosomal protein 170B) (Cep170B),FUNCTION: Plays a role in microtubule organization. {ECO:0000250|UniProtKB:Q5SW79}.

Molecular Weight: 170.8 kDa

UniProt: Q80U49