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TCTN1 Protein (AA 23-593) (His tag)

Crystallography grade TCTN1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3136326
$16,803.00
Plus shipping costs $45.00 and $20.00 dry ice
Shipping to: United States
Will be delivered in 60 Business Days
  • Target See all TCTN1 Proteins
    TCTN1 (Tectonic Family Member 1 (TCTN1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 23-593
    Origin
    Mouse
    Source
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This TCTN1 protein is labelled with His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    QVIAIPAAAT PAVTKEDLNS TKATPTTLQP SLSPRTPGTP RAPERSGPRP TPVTDVAALC VCDLLPAQCD VNCCCDPDCS PADFSIFSAC SVPVVTGDRQ FCSQKAAFYS MNLTADPPHR DFKLIDQINP SVFCIHISNY KPALSFANPE VPDENNFDRL MQTSGGFTLS AESAVPSTAA SDGPQPTKYE YGAPLQTAGA SSGSFLKLPS PLTSSLCADQ NPAAFLVSQA FECSRRVDIE QCEGMEALSM AHYSSPAILR VPNSMTQVSI KIQSVMYRSL NHTLTQLEGH GVLRPSLVST GQDRLCSNVV LQVKYSLLYT ATGQIHEAGL SLVLGTLSSA VSLLQQKFEI HFIQHGTKPV PLSGNPGYRV GLPLAAGFQP QKGSGIIQTT NRQGQFTILR STSQQDCLAS EGLRTPVLFG YNVQSGCQLR LTGTIPCGLL AQKVQDLLRG QAFPDYVAAF GNSRAQDVQD WVPVHFVTYS SNMKGSCQLP VALAIEVKWT KYGSLLNPQA RIVNVTAQLV SVPEPLPGPE RTVVISTAVT FVDVSAPAEA GFRAPPTINA RLPFSFFFPF V
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Tctn1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    TCTN1 (Tectonic Family Member 1 (TCTN1))
    Alternative Name
    Tctn1 (TCTN1 Products)
    Synonyms
    FLJ21127 Protein, JBTS13 Protein, TECT1 Protein, G730031O11Rik Protein, Tect1 Protein, RGD1566266 Protein, tectonic family member 1 Protein, tectonic family member 1 L homeolog Protein, TCTN1 Protein, tctn1.L Protein, tctn1 Protein, Tctn1 Protein
    Background
    Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Regulator of Hedgehog (Hh), required for both activation and inhibition of the Hh pathway in the patterning of the neural tube. During neural tube development, it is required for formation of the most ventral cell types and for full Hh pathway activation. Functions in Hh signal transduction to fully activate the pathway in the presence of high Hh levels and to repress the pathway in the absence of Hh signals. Modulates Hh signal transduction downstream of SMO and RAB23. {ECO:0000269|PubMed:16357211, ECO:0000269|PubMed:21725307, ECO:0000269|PubMed:22179047}.
    Molecular Weight
    62.1 kDa Including tag.
    UniProt
    Q8BZ64
    Pathways
    Tube Formation
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