CAMKK2 Protein (AA 2-588) (His tag)

Catalog No. ABIN3136339

Product Details

Target: CAMKK2 (Calcium/calmodulin-Dependent Protein Kinase Kinase 2, beta (CAMKK2))

Protein Type: Recombinant

Protein Characteristics: AA 2-588

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This CAMKK2 protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: SSCVSSQPTS DRVAPQDELG SGGGSREGQK PCEALRGLSS LSIHLGMESF IVVTECEPGR GVDLNLARDQ PPEADGQELP LEASDPESRS PLSGRKMSLQ EPSQGGPASS SNSLDMNGRC ICPSLSYSPA SSPQSSPRMP RRPTVESHHV SITGLQDCVQ LNQYTLKDEI GKGSYGVVKL AYNENDNTYY AMKVLSKKKL IRQAGFPRRP PPRGARPAPG GCIQPRGPIE QVYQEIAILK KLDHPNVVKL VEVLDDPNED HLYMVFELVN QGPVMEVPTL KPLSEDQARF YFQDLIKGIE YLHYQKIIHR DIKPSNLLVG EDGHIKIADF GVSNEFKGSD ALLSNTVGTP AFMAPESLSE TRKIFSGKAL DVWAMGVTLY CFVFGQCPFM DERIMCLHSK IKSQALEFPD QPDIAEDLKD LITRMLDKNP ESRIVVPEIK LHPWVTRHGA EPLPSEDENC TLVEVTEEEV ENSVKHIPSL ATVILVKTMI RKRSFGNPFE GSRREERSLS APGNLLTKKP TREWEPLSEP KEARQRRQPP GPRAGPCGGG GSALVKGGPC VESWGAPAPG SPPRMPPLQP EEVMEPE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Camkk2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CAMKK2 (Calcium/calmodulin-Dependent Protein Kinase Kinase 2, beta (CAMKK2))

Alternative Name: Camkk2 (CAMKK2 Products)

Synonyms: CAMKK Protein, CAMKKB Protein, 6330570N16Rik Protein, AW061083 Protein, mKIAA0787 Protein, camk2b Protein, fj90d04 Protein, wu:fj90d04 Protein, si:dkey-174n20.2 Protein, CAMKK2 Protein, calcium/calmodulin dependent protein kinase kinase 2 Protein, calcium/calmodulin-dependent protein kinase kinase 2, beta Protein, calcium/calmodulin-dependent protein kinase (CaM kinase) II beta 1 Protein, calcium/calmodulin dependent protein kinase II beta Protein, calcium/calmodulin-dependent protein kinase kinase 2 Protein, CAMKK2 Protein, Camkk2 Protein, camk2b1 Protein, CAMK2B Protein, camkk2 Protein

Background: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1, CAMK4 and CAMK1D (By similarity). Efficiently phosphorylates 5'-AMP-activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals (By similarity). May play a role in neurite growth. Isoform 2 may promote neurite elongation, while isoform 1 may promoter neurite branching (By similarity). May be involved in hippocampal activation of CREB1. {ECO:0000250, ECO:0000269|PubMed:14586002}.

Molecular Weight: 65.4 kDa Including tag.

UniProt: Q8C078

Pathways: AMPK Signaling