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Coiled-Coil and C2 Domain Containing 2A (CC2D2A) (AA 1-1633) protein (His tag) Protein

Crystallography grade CC2D2A Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3136440
$15,464.90
Plus shipping costs $45.00 and $24.00 dry ice
1 mg ABIN3136440
1 mg ABIN3136440
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Will be delivered in 70 Business Days
  • Target
    CC2D2A
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1633
    Origin
    Mouse
    Source
    • 1
    Insect Cells
    Purification tag / Conjugate
    This CC2D2A protein is labelled with His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MNTNDEKMKI ISEDFTGDGV DTEAGRRKKN SKVRRQQRKK KPPASPPEEM VSDKFQDGGQ QEVVEEEPET NLLSLTARRG PRSLPPIPSA SRTGFAEFSM RERMREKLQA ARSKAESALL RDVPTPRPRR LRSPSREKET ETEFGTEPST EVQDTQKEDD TKSYSRIKFR DSVRKIKSKP QLPPGFPSAE EAYNFFTFNF DPEPEESEEK SPVKGGERAH HEDQEGEEGT QAQERAKKTE EEELLNGKDA EDFLLGLDPT AHDFVAVRAA EYKSARIQLQ KEKEILFTPS RLTVPTYKKL PENIQPRFLE DEGLYIGARP EVARTNENIM ENRLLIQEPG SKWFGDDGRI LALPSPIKPF PSRPSLTTRE QSPKAGLETL YKKAEKYVHS RQHMIGSGDP PGNFQLDIDI SGLIFTHHPC FSREHVLASK LAQLYDQYLA RQQRNKTKFL TDKLQALRKA VQTSLNPEKP HQSLDTTQKT INEYKSEIRQ TRKLRDAEQE KDRTLLKTII KVWKEMKSLR EFQRFTNTPL KLVLRKEKVD PKLDEDAYEA EIQAEIHELL EEHMEEYATK MEEYRTSHQQ WKAWRKAQRA KKKKKKQTTE EHLEEEEAEE SFPEEEVTKP IPPEPTDPAV IEQQVRERAA HSRRRPGEPT LIPELSLAGN VTPNDQCPRV EVSRREDVRR RSVYLKVVFN SKEVSRTVSR PLGADFRVHF GQIFNLQIFN WPESLMLQVY ETIGHSGTTL LAEVFLPIPE TTLVTGRAPI EEVEFSSNQH VTLDHEGVGS GVPFSFEADG SNQLTLMTSG KVSHSVAWAV GENGIPLIPP LSQQNIGFRS ALRRADAISS IGTSGLTDMK KLAKWAAESK LDPNDPNHAP LMQLISVATS GESYVPDFFR LEQLQQEFNF VSEEELNRSK RFRLLHLRSQ EVPEFRNYKQ IPAYDREIME KVFQDYEKRL RDRNVIETKD HLDMHRATVA KYLQQVREAV VNRFLTAKHH FLLTDLVVEE EVPNISSEGS GILGLSLFKL AEQKRPLRPR RKGRKKVTAQ NLSDGDIKLL VNIIRAYDIP VRKPVVSKFQ QPSRSSRTFS EKQTASPSTH SPLHNADYPL GQVLVRPFVE VSFQRTICHT TTAEGPNPSW NEELELPFRA PNGDYSTASL QSVKDDVYIN IFDEVLYDIL EDDRERGSGI HTRIERHWLG CVKIPFSTIY FQARIDGTFK IDIPPVLLGY SKERNIIMER AFDSARSLSE GSYITLFITI EPQLVPGEPM REKMSDMLKK FDTQEDEKLL QATEKFQAEC ALKFPQRQCL TTVTDMTGKT VFITRYLKPL NPPQELLHVY PNNPQATAEL VARYVSLIPF LPDSVSFAGV CDLWSTSDQF LDLLAGDEEE HAVLLCNYFL FLGKKAWLVM GSAIPEGPTA YVLTWEKNYY LIWNPCSGHC YGQFDAFCPL KSVGCLIGPD NIWFNIQHHD SPLRINFDVT KPKLWKSFFS RSLPYPGLSS VQPEELIYQH TDKAVAAELQ DRIEKILKEK IMDWRPRHLT RWNRYCTSTL RHFLPLLERS QGEDIEDDHR AELLKQLGDY RFSGFPLHMP YSEVKPLVEA VYSTGVHNID LPNVEFALAV YIHPYPKNVL SVWIYVASLV RNR
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Cc2d2a Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    CC2D2A
    Synonyms
    JBTS9, MKS6, 5730509K17Rik, b2b1035Clo, fc03c12, wu:fc03c12, RGD1561042, coiled-coil and C2 domain containing 2A, coiled-coil and C2 domain containing 2A L homeolog, CC2D2A, Cc2d2a, cc2d2a.L, cc2d2a
    Background
    Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Required for ciliogenesis and sonic hedgehog/SHH signaling. {ECO:0000269|PubMed:21725307, ECO:0000269|PubMed:22179047}.
    Molecular Weight
    188.5 kDa Including tag.
    UniProt
    Q8CFW7
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