RPGRIP1L Protein (AA 1-1264) (Strep Tag)

Catalog No. ABIN3153902

Product Details

Target: RPGRIP1L (RPGRIP1-Like (RPGRIP1L))

Protein Type: Recombinant

Protein Characteristics: AA 1-1264

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This RPGRIP1L protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MSGPSDETAG DLPVKDTGLN LFGVGGLQET STARTVKTRQ AVSRVSREEL EDRFLRLHDE NILLKQHARK QEDKIKRMAT KLIRLVNDKK RYERVGGGPK RLGRDVEMEE MIEQLQEKVH ELERQNEVLK NRLISAKQQL QVQGHRQTSY SRVQARVNTG RRRASASAGS QECPGKGLRF QNVDEAETVQ PTLTKYSNSL LEEARGEIRN LENVIQSQRG QIEELEHLAE ILKTQLKRKE NEIELSLLQL REQQATDQRS NIRDNVETIK LHKQLVEKSN ALSVIEGKFI QLQEKQRTLR ISHDALMANG DELNKQLKEQ RLKCCSLEKQ LHSVRFSERR VEELQDRIND LEKERELLKE NYDKLYNSAF SAAHEEQWKL KEQQMKVQIA QLETALKSDL TDKTEVLDKL KTERDQNEKL VQENRDLQLQ CLQQKQRLHE LQSRLKFFNQ ESDINADDLS EALLLIKAQK EQKNGDLSFL EKVDSKINKD LDRSMKELQA THAETVQELE KTRNMLIMQH KINKDYQMEV ETVTQKMENL QQDYELKVEQ YVHLLDIRAA RIQKLEAQLK DIAYGTKQYK FKPEIMPDDS VDEFDETIHL ERGENLFEIH INKVTFSSEV LRASGDKELV TFCTYAFYDF ELQTTPIVRG LYPEYNFTSQ YLVHVNDLFL QYIQKNTVTL ELHQAHSTDY ETIAACQLRF HEILEKSGRI FCTTSLVGTK GDIPNFGTVE YWFRLRVPMD QAIRLYRERA KALGYITSNF KKPEKMQLSS QQAATTAQIS PAESTDGNLN ELHVTVKCCT GLQSRASYLQ PHAYVVYKFF DFPDHDTAIV PSSNDPQFDD HMCFPVPMNM DLDRYLKSES LSFYVFDDSD TQENIYMGKV NVPLISLAHD KCISGIFELM DKEKHAAGTI QVILKWKFTY LPPSGSITTE DLGKFVCREE PEAVQRLPPK SSDVTSVVAP KPKPRQRLTF VDKKVSFADT ISHPSPETSP PPKDIKDSSP EVGPKPENGL SAVAYPSKES GVAKVEENVG EMQQGKEDDI SFLSEGQLAS GSVASSEDET EITEELEPED EDRSASDSDD CIIPSSVSTN TKQPSEEIRI EIIALNLNDS QITREDTIQR LFIECRFYSL PAEETPMSLP KPQSGQWVYY NYSNVIYLDK ENNPAVRDIL KAILQRRELP HRSVRFTVVS DPPEDEQDLE CEDIGVAHVD LADLFQKGRD IIEQDIDVLD ARTDGGTIGK LKVTVEALHA LRSVYEQNRK DLEA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RPGRIP1L (RPGRIP1-Like (RPGRIP1L))

Alternative Name: Rpgrip1l (RPGRIP1L Products)

Synonyms: si:ch1073-301i20.1 Protein, mks5 Protein, cors3 Protein, jbts7 Protein, nphp8 Protein, CORS3 Protein, FTM Protein, JBTS7 Protein, MKS5 Protein, NPHP8 Protein, 1700047E16Rik Protein, 4931437C01 Protein, Ftm Protein, Nphp8 Protein, RGD1311099 Protein, RPGRIP1 like Protein, RPGRIP1-like Protein, Protein fantom Protein, Rpgrip1-like Protein, RPGRIP1L Protein, rpgrip1l Protein, mks-5 Protein, Rpgrip1l Protein

Background: Protein fantom (Nephrocystin-8) (RPGR-interacting protein 1-like protein) (RPGRIP1-like protein),FUNCTION: Negatively regulates signaling through the G-protein coupled thromboxane A2 receptor (TBXA2R) (By similarity). May be involved in mechanisms like programmed cell death, craniofacial development, patterning of the limbs, and formation of the left-right axis. Involved in the organization of apical junctions, the function is proposed to implicate a NPHP1-4-8 module. Does not seem to be strictly required for ciliogenesis (By similarity). Involved in establishment of planar cell polarity such as in cochlear sensory epithelium and is proposed to implicate stabilization of disheveled proteins (PubMed:22927466). Involved in regulation of proteasomal activity at the primary cilium probably implicating association with PSDM2 (PubMed:26150391). {ECO:0000250|UniProtKB:Q68CZ1, ECO:0000269|PubMed:10501967, ECO:0000269|PubMed:11956760, ECO:0000269|PubMed:21565611, ECO:0000269|PubMed:22927466, ECO:0000269|PubMed:26150391}.

Molecular Weight: 145.0 kDa

UniProt: Q8CG73

Pathways: DNA Replication, Regulation of G-Protein Coupled Receptor Protein Signaling, Synthesis of DNA