CCAR1 Protein (AA 1-1146) (Strep Tag)

Catalog No. ABIN3136465

Product Details

Target: CCAR1 (Cell Division Cycle and Apoptosis Regulator 1 (CCAR1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1146

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This CCAR1 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MAQFGGQKNP PWATQFTATA VSQPAALGVQ QPSLLGASPT IYTQQTALAA AGLTTQTPAN YQLTQTAALQ QQAAAVLQQQ YSQPQQALYS VQQQLQQPQQ TILTQPAVAL PTSLSLSTPQ PAAQITVSYP TPRSSQQQTQ PQKQRVFTGV VTKLHDTFGF VDEDVFFQLG AVKGKTPQVG DRVLVEATYN PNMPFKWNAQ RIQTLPNQNQ SQTQPLLKTP TAVIQPIVPQ TTFGVQAQPQ PQSLLQAQIS AASITPLLQT QPQPLLQQPQ QKAGLLQPPV RIVSQPQPAR RLDPPSRFSG RNDRGDQVPN RKDDRSRERD RERRRSRERS PQRKRSRERS PRRERERSPR RVRRVVPRYT VQFSKFSLDC PSCDMMELRR RYQNLYIPSD FFDAQFTWVD AFPLSRPFQL GNYCNFYVMH REVESLEKNM AVLDPPDADH LYSAKVMLMA SPSMEDLYHK SCALAEDPQD LRDGFQHPAR LVKFLVGMKG KDEAMAIGGH WSPSLDGPNP EKDPSVLIKT AIRCCKALTG IDLSVCTQWY RFAEIRYHRP EETHKGRTVP AHVETVVLFF PDVWHCLPTR SEWETLSRGY KQQLVEKLQG ERKKADGEQD EEEKDDGEVK EIATPTHWSK LDPKAMKVND LRKELESRAL SSKGLKSQLI ARLTKQLKIE EQKEEQKELE KSEKEEEDED DKKSEDDKEE EERKRQEEVE RQRQERRYIL PDEPAIIVHP NWAAKSGKFD CSIMSLSVLL DYRLEDNKEH SFEVSLFAEL FNEMLQRDFG VRIYKSLLSL PEKEDKKDKE KKSKKEERKD KKEEREDDID EPKPKRRKSG DDKDKKEDRD ERKKEEKRKD DSKDDDETEE DNNQDEYDPM EAEEAEDEDD DREEEEVKRD DKRDVSRYCK DRPAKDKEKE KPQMVTVNRD LLMAFVYFDQ SHCGYLLEKD LEEILYTLGL HLSRAQVKKL LNKVVLRESC FYRKLTDTSK DDENHEESEA LQEDMLGNRL LLPTPTIKQE SKDGEENVGL IVYNGAMVDV GSLLQKLEKS EKVRAEVEQK LQLLEEKTDE DGKTILNLEN SNKSLSGELR EVKKDLGQLQ ENLEVSENMN LQFENQLNKT LRNLSTVMDD IHTVLKKDNV KSEDRDEKSK ENGSGV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CCAR1 (Cell Division Cycle and Apoptosis Regulator 1 (CCAR1))

Alternative Name: Ccar1 (CCAR1 Products)

Synonyms: CCAR1 Protein, KIAA1967 Protein, ccar1 Protein, MGC81663 Protein, zehn1480 Protein, hm:zehn1480 Protein, si:dkey-85g17.2 Protein, RP11-437A18.1 Protein, CARP-1 Protein, 2610511G16Rik Protein, 9430036H15Rik Protein, Carp1 Protein, RGD1560358 Protein, cell division cycle and apoptosis regulator 1 Protein, cell division cycle and apoptosis regulator 1 L homeolog Protein, cell division cycle and apoptosis regulator protein 1 Protein, CCAR1 Protein, ccar1.L Protein, LOC5575286 Protein, ccar1 Protein, LOC409891 Protein, Ccar1 Protein

Background: Cell division cycle and apoptosis regulator protein 1 (Cell cycle and apoptosis regulatory protein 1) (CARP-1),FUNCTION: Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation. May be involved in apoptosis signaling in the presence of the retinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation (By similarity). In association with CALCOCO1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781). Can act as a both a coactivator and corepressor of AR-mediated transcription. Contributes to chromatin looping and AR transcription complex assembly by stabilizing AR-GATA2 association on chromatin and facilitating MED1 and RNA polymerase II recruitment to AR-binding sites. May play an important role in the growth and tumorigenesis of prostate cancer cells (PubMed:23887938). {ECO:0000250|UniProtKB:Q8IX12, ECO:0000269|PubMed:23887938, ECO:0000269|PubMed:24245781}.

Molecular Weight: 132.1 kDa

UniProt: Q8CH18