Myocardin Protein (MYOCD) (AA 1-935) (Strep Tag)

Catalog No. ABIN3136770

Product Details

Target: Myocardin (MYOCD)

Protein Type: Recombinant

Protein Characteristics: AA 1-935

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This Myocardin protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MTLLGSEHSL LIRRKFRSVL QLRLQQRRTQ EQLANQGLIP PLKGPTEFHD PRKQLDSAKT EDSLRRKGRN RSDRASLVTM HILQASTAER SIPTAQMKLK RARLADDLNE KIALRPGPLE LVEKNILPMD SSVKEAIKGT EVSLSKAADA FAFEDDSSRD GLSPDQARSE DPQGSTGSTP DIKSTEAPLD TIQDLTPGSE SDKNDAASQP GNQSDPGKQV LGPLSTPIPV HTAVKSKSLG DSKNRHKKPK DPKPKVKKLK YHQYIPPDQK AEKSPPPMDS AYARLLQQQQ LFLQLQILSQ QQQQQQQQQQ QQQQQQQQQQ RFSYPGMHQT HLKEPNEQMA RNPNPSSTPL SNTPLSPVKN SISGQTGVSS LKPGPLPPNL DDLKVSELRQ QLRIRGLPVS GTKTALVDRL RPFQDCAGNP VPNFGDITTV TFPVTPNTLP SYQSSPTGFY HFGSTSSSPP ISPASSDLSA AGSLPDTFTD ASPGFGLHAS PVPACTDESL LSSLNGGSGP SEPDGLDSEK DKMLVEKQKV INQLTWKLRQ EQRQVEELRM QLQKQKSSCS DQKPLPFLAT TIKQEDVSSC PFAPQQASGK GQGHSSDSPP PACETAQLLP HCVESSGQTH VLSSTFLSPQ CSPQHSPLGG LKSPQHISLP PSPNNHYFLA SSSGAQRENH GVSSPSSSQG CAQMTGLQSS DKVGPTFSIP SPTFSKSSSA VSDITQPPSY EDAVKQQMTR SQQMDELLDV LIESGEMPAD AREDHSCLQK IPKIPGSSCS PTAIPPKPSA SFEQASSGGQ MAFDHYANDS DEHLEVLLNS HSPIGKVSDV TLLKIGSEEP PFDSIMDGFP GKAAEDLFSA HELLPGPLSP MHAQLSPPSV DSSGLQLSFT ESPWETMEWL DLTPPSSTPG FSNLTSSGPS IFNIDFLDVT DLNLNSPMDL HLQQW
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Myocardin (MYOCD)

Alternative Name: Myocd (MYOCD Products)

Synonyms: myocd Protein, MYOCD Protein, MYCD Protein, BSAC2A Protein, Srfcp Protein, Mycd Protein, myocardin Protein, myocardin L homeolog Protein, MYOCD Protein, myocd.L Protein, Myocd Protein

Background: Myocardin (Basic SAP coiled-coil transcription activator 2) (SRF cofactor protein),FUNCTION: Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis, urinary bladder development, and differentiation of the smooth muscle cell lineage (myogenesis). Positively regulates the transcription of genes involved in vascular smooth muscle contraction (By similarity). {ECO:0000250|UniProtKB:Q8R5I7, ECO:0000269|PubMed:11439182, ECO:0000269|PubMed:12640126, ECO:0000269|PubMed:12663482, ECO:0000269|PubMed:16818234, ECO:0000269|PubMed:20385216, ECO:0000269|PubMed:31513549}., FUNCTION: [Isoform 1]: Positively regulates the activation of smooth muscle cell gene promoter regions. {ECO:0000269|PubMed:20385216}., FUNCTION: [Isoform 3]: Positively regulates the activation of smooth muscle cell gene promoter regions (PubMed:20385216). Activation of the MYH6 promoter is enhanced in the presence of MEF2C (PubMed:20385216). {ECO:0000269|PubMed:20385216}., FUNCTION: [Isoform 4]: Positively regulates the activation of smooth muscle cell gene promoter regions. {ECO:0000269|PubMed:20385216}., FUNCTION: [Isoform 5]: Positively regulates the activation of smooth muscle cell gene promoter regions. {ECO:0000269|PubMed:20385216}.

Molecular Weight: 101.4 kDa

UniProt: Q8VIM5

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development