RIN1 Protein (AA 1-763) (Strep Tag)

Catalog No. ABIN3136876

This Recombinant RIN1 protein is expressed in Cell-free protein synthesis (CFPS).

Quick Overview for RIN1 Protein (AA 1-763) (Strep Tag) (ABIN3136876)

Target: RIN1 (Ras and Rab Interactor 1 (RIN1))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-763

Purification tag / Conjugate: This RIN1 protein is labelled with Strep Tag.

Sequence: MEDPGETGAH PLGATNLNFV PGHQQKEKPS TDPLYDTPDT RGVQAGGSQQ PARTVSLRER LLITRPVWLQ LRANAAAALH VLRTEPPGTF LVRKSNTRQC QALCVRLPEA SGPSFVSSHY IEESTGGVSL EGSELMFQDL VQLICGYCRT RAIHQAATHK ELEAISHLGM EFWSSSLNTK DQQRPSEAPP IPRLKARSPQ ELDQGTGAAL CFFNPLFPGD LGPTKREKFK RSFKVRVSTE TSSPLSPPAV PPPPVPVLPG TSSSQTERLP PRQLLQRESS VGYRVPGSAA SPCLPPLPSL QEVDCCSPSS SEEEGSSGSP TTSPRLSRPR HRRPLLRSMS SAFCSLLAPE RQVGRAATML MQNRYTAVGQ LVQDLLTQVR AGPEPRELQG IRQALSRARA MLSAELGPEK LLPPERLELV LEKSLHRSVL KPLRPILAAR LRRRLSADGS LGRLAEGFRL ARTQGPGAFG SHLTLSSPVE TEQVRQKLLQ LLRAYSPSAQ VKWLLQACKL LYTALKSQAG ENAGADEFLP LLSLVLAQCD LPDLLLEAEY MSELLEPTLL TGEGGYYLTS LSASLALLSG LSQARALPLS PAQELQRSLA LWEQRRLPAT HSFQHLLRVA YQDPSTGCTS KTLAVPPGSS IATLSQLCAT KFRVTQPDAF GLFLYKDQGY HRLPPEALAH RLPATGYLIY RRAERPETQG AVAEKAKTGS KGPEAGAWEE ETGGLNREGK PRIAVDQEGK DQARGGHIGP EEQKAEGSQA LEE
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RIN1 (Ras and Rab Interactor 1 (RIN1))

Alternative Name: Rin1

Background: Ras and Rab interactor 1 (Ras interaction/interference protein 1),FUNCTION: Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF) for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis (By similarity). {ECO:0000250, ECO:0000269|PubMed:12574403}.

Molecular Weight: 83.0 kDa

UniProt: Q921Q7