SLC43A2 Protein (AA 1-568) (Strep Tag)

Catalog No. ABIN3125999

Product Details

Target: SLC43A2 (Solute Carrier Family 43, Member 2 (SLC43A2))

Protein Type: Recombinant

Protein Characteristics: AA 1-568

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SLC43A2 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MAPTLATAHR RRWWMACTAV LENLLFSAVL LGWGSLLIML KSEGFYSYLC TKPENVTNST VGGSAEPEPE ELSLVNGWLS CKAQDEILNL AFTVGSFLLS AITLPLGIIM DKYGPRKLRL LGSACFAVSC LLIAYGASNP DSLSVLIFIA LALNGFGGMC MTFTSLTLPN MFGDLRSTFI ALMIGSYASS AVTFPGIKLI YDAGASFIGI LVVWAGCSGL VFFNCFFNWP LEPFPGPEDM DYSVKIKFSW LGFDHKITGK QFYKQVTTVG RRLSVGSSMR TAKEQAALQE GHKLCLSTVD LEVKCQPDAA AAPSFMHSVF SPLLVLSLVT MCVTQLRLIF YMGAMNSILE FLVRGDQKTV ALYTSIFGAL QLLCLLTAPV IGYIMDWKLK ECEDTSEEPE EKEGTQGEKK QKRDRQIQKV TNAMRAFAFT NVLLVGFGVT CLIPNLPLQI FSFVLHTIVR GFIHSAVGGL YAAVYPSTQF GSLTGLQSLV SALFALLQQP LYLAMMGPLG GDPLWVNVGL LAMSMLGFCL PLYLICYRRQ LERQLQQKRE DSKLFLKING SSNREAFV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SLC43A2 (Solute Carrier Family 43, Member 2 (SLC43A2))

Alternative Name: Slc43a2 (SLC43A2 Products)

Synonyms: LAT4 Protein, lat4 Protein, MGC122003 Protein, 7630402D21Rik Protein, BC042513 Protein, Lat4 Protein, RGD1305819 Protein, si:dkey-118k5.2 Protein, slc43a2 Protein, zgc:56271 Protein, DKFZp469D1521 Protein, cb941 Protein, wu:fb37c04 Protein, wu:fb38e03 Protein, wu:fb52a10 Protein, wu:fi32b04 Protein, xx:lithzf000079 Protein, zgc:103664 Protein, solute carrier family 43 member 2 Protein, solute carrier family 43 (amino acid system L transporter), member 2 Protein, solute carrier family 43, member 2 Protein, solute carrier family 43 (amino acid system L transporter), member 2a Protein, solute carrier family 43 (amino acid system L transporter), member 2 L homeolog Protein, solute carrier family 43 (amino acid system L transporter), member 2b Protein, SLC43A2 Protein, slc43a2 Protein, Slc43a2 Protein, slc43a2a Protein, slc43a2.L Protein, slc43a2b Protein

Background: Large neutral amino acids transporter small subunit 4 (L-type amino acid transporter 4) (Solute carrier family 43 member 2),FUNCTION: Uniporter that mediates the transport of the stereospecific L-phenylalanine, L-methionine and L-branched-chain amino acids, between the extracellular space and the cytoplasm and may control the transepithelial (re)absorption of neutral amino acid in kidney and small intestine (PubMed:25480797, PubMed:15659399). Transport activity is mediated through facilitated diffusion and is sodium ions-, chloride ions- and pH -independent (PubMed:25480797). {ECO:0000269|PubMed:15659399, ECO:0000269|PubMed:25480797}.

Molecular Weight: 62.4 kDa

UniProt: Q8CGA3