SLC4A11 Protein (AA 1-862) (Strep Tag)

Catalog No. ABIN3146935

Product Details

Target: SLC4A11 (Solute Carrier Family 4, Sodium Borate Transporter, Member 11 (SLC4A11))

Protein Type: Recombinant

Protein Characteristics: AA 1-862

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SLC4A11 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MSQNEHCQDS GEYFSAGTQG YFKNNMEDNL EVREDSLGDE VFDTVNSSIV SGESIRFFVN VNLEVQPSKS DLEAATGGCV LLHTSRKYLK LKNFEEEVRA HRDLDGFLAQ ASIILNETAT SLDDVLRTML NRFALDPNHA EPDCDLDLLM AKLFTDAGAP MESKVHLLSD TIQGVTATVR GVQYEQSWLC IICTMKTLQK RHVCISRLVR PQNWGENSCE VRFVILVLAP PKMKSTKTAM EVARTFATMF SDITFRQKLL KTRTEEEFKE ALVHQRQLLT MMMPRAAGHS MSSLHTHRHP QPPKCKDFFP FGKGIWMDIM RRFPVYPMDF TDGIIGKSKS VGKYVTTTLF LYFACLLPTI AFGSLNDENT NGAIDVQKTI AGQSIGGLLY ALFSGQPLVI LLTTAPLAIY TQVIRVICDD YNLDFNAFYA WTGLWNSFFL ALYAFLNLSL LMNLFKRSTE EIIALFISIT FVLDAVKGMV KIFGKYYYGH HYHTKRTSSL VSLLGIGRSP NSSLHTALNA SLLASPVEMA TTSSPGSTHS GQATAVLSLL IMLGTLWLGY TLYQFKKSPY LHPCVRETLS DCALPIAVLS FSLIGSYGFQ EIEMSKFRYN PSESLFEVAQ IHSLSFKAIG SAMGLGFLLS LLFFIEQNLV AALVNAPENR LVKGTAYHWD LLLLAIINTG LSLFGLPWIH AAYPHSPLHV RALALVEERV ENGHIYETIV DVKETRLTAL GASVLVGLSL LLLPFPLQWI PKPVLYGLFL YIALTSLDGN QLFSRVALLL KEQTSYPPTH YIRRVPQRKI HYFTGLQILQ LLLLCAFGMS SLPYMKMVFP LIMIAMIPIR YNLLPRIIEA KYLDVMDAEH RP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SLC4A11 (Solute Carrier Family 4, Sodium Borate Transporter, Member 11 (SLC4A11))

Alternative Name: Slc4a11 (SLC4A11 Products)

Synonyms: NaBC1 Protein, si:dkey-12j14.4 Protein, AI503023 Protein, BTR1 Protein, CDPD1 Protein, CHED2 Protein, NABC1 Protein, dJ794I6.2 Protein, solute carrier family 4 member 11 Protein, solute carrier family 4 member 11 L homeolog Protein, solute carrier family 4, sodium borate transporter, member 11 Protein, solute carrier family 4, sodium bicarbonate transporter-like, member 11 Protein, Slc4a11 Protein, SLC4A11 Protein, slc4a11.L Protein, slc4a11 Protein

Background: Solute carrier family 4 member 11 (Sodium borate cotransporter 1) (NaBC1),FUNCTION: Multifunctional transporter with an impact in cell morphology and differentiation (PubMed:20185830). In the presence of borate B(OH)4(-), acts as a voltage-dependent electrogenic Na(+)-coupled B(OH)4(-) cotransporter controlling boron homeostasis (By similarity). At early stages of stem cell differentiation, participates in synergy with ITGA5-ITGB1 and ITGAV-ITGB3 integrins and BMPR1A to promote cell adhesion and contractility that drives differentiation toward osteogenic commitment while inhibiting adipogenesis (PubMed:33247189). In the absence of B(OH)4(-), acts as a Na(+)-coupled OH(-) or H(+) permeable channel with implications in cellular redox balance. Regulates the oxidative stress response in corneal endothelium by enhancing antioxidant defenses and protecting cells from reactive oxygen species. In response to hypo-osmotic challenge, also acts as water permeable channel at the basolateral cell membrane of corneal endothelial cells and facilitates transendothelial fluid reabsorption in the aqueous humor. In the presence of ammonia, acts as an electrogenic NH3/H(+) cotransporter and may play a role in ammonia transport and reabsorption in renal Henle's loop epithelium (By similarity). {ECO:0000250|UniProtKB:Q8NBS3, ECO:0000269|PubMed:20185830, ECO:0000269|PubMed:33247189}.

Molecular Weight: 96.8 kDa

UniProt: A2AJN7

Pathways: Proton Transport