DENND5B Protein (AA 2-1274) (rho-1D4 tag)

Catalog No. ABIN3147027

Product Details

Target: DENND5B (DENN/MADD Domain Containing 5B (DENND5B))

Protein Type: Recombinant

Protein Characteristics: AA 2-1274

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This DENND5B protein is labelled with rho-1D4 tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: SGSSAAPGPG SGSSPAACRF AHYFVLCGID ADSGLEPDEL AGENFDQSPL RRTFKSKVLA HYPQNIEWNP FDQDAVNMLC MPKGLSFRTQ ADNKEPQFHS FIITREDGSR TYGFVLTFYE EVTSKQICTA MQTLYQMHNA EQYSSVYASS SCSMDSLASS IDEGDATSLL KLQRYNSYDI NRDTLYVSKS ICLITPLPFM QACKKFLFQL HKAVTSQQPP PLPLESYIHN ILYEVPLPPP GRSLKFYGVY EPVICQRPGP NELPLSDYPL REACELLGLE NLVQVFTCVL LEMQTLLYSQ DYQRLMTVAE GITTLLFPFQ WQHVYVPILP ASLLHFLDAP VPYLMGLQSK EGTDRSKLEL PQEANLCFVD IDNHFIELPE EFPQFPNKVD FIQELSEVLL QFGIPPEGSL HSSESATKLK NMVLKDLAND KKNGNVPNNS VSVYELLKGS ETIARLQALA KRTGVTMEKI DLPASLSEKE KDLKLQCEEA DLRDSQLNVQ LREVFANRFT QMFADYEAFV IQTAQDMESW LTNREQMQNF DKASFLSDQP EPYLPFLSRF IETQMFATFI DNKIMSQWEE KDPLLRVFDS RIEKIRLYNV RAPTLRTSIY QKCSSLKEAA QSIEQRLMKM DHTAIHPHLL DMKIGQGKYE QGFFPKLQSD VLATGPANNN RWVSRSATAQ RRKERLRQSS EHIGLDSDLR EKYMQEARSL GKNLRQPKLS DLSPAVIAQT NWKFVEGLLK ECRMKTKRML VEKMGHEAVE LGHGEANITG LEENTLIASL CDLLERIWSH GLLVKQGKSA LWSHLLQFQD REEKQEHLTD SPVALGPERR KSDSGVMLPT LRVSLIQDMR HIQNMTEIKT DVGRARAWIR LSLEKKLLSQ HLKQLLSNQP LTKKLYKRYA FLRCEEEREQ FLYHLLSLNA VDYFCFTSVF TTIMIPYRSV IIPIKKLSNA IITSNPWICV SGELGDTGVM QIPKNLLEMT FECQNLGKLT TVQIGHDNSG LLAKWLVDCV MVRNEITGHT YRFPCGRWLG KGVDDGSLER ILIGELMTSA SDEDLGKQCR TPPQQKSPTT TRRLSITSLT GKPAKPNAGQ IQEGIGEAVN NIVKHFHKPE KERGSLTVLL CGENGLVAAL EQVFHHGFKS ARIFHKNVFI WDFVEKAVAY FETTDQILDN EGDVLIQKPS SKTFCHYVNA INTAPRNIGK DGKFQILVCL GTRDHLLPQW IPLLAECPAI TRMYEENALL RDHMTVNSLI RILQTIQDFT IVLEGSLIKG VDV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Dennd5b Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: DENND5B (DENN/MADD Domain Containing 5B (DENND5B))

Alternative Name: Dennd5b (DENND5B Products)

Synonyms: 9330160C06Rik Protein, AI852444 Protein, D030011O10Rik Protein, ENSMUSG00000072657 Protein, Gm17524 Protein, DENN domain containing 5B Protein, DENN/MADD domain containing 5B Protein, DENND5B Protein, Dennd5b Protein

Background: Guanine nucleotide exchange factor (GEF) which may activate RAB39A and/or RAB39B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form (By similarity). {ECO:0000250}.

Molecular Weight: 145.7 kDa Including tag.

UniProt: A2RSQ0