Immunoglobulin Superfamily, Member 9B (IGSF9B) (AA 18-1328) protein (rho-1D4 tag)

Details for Product No. ABIN3147301
Protein Name
Protein Characteristics
AA 18-1328
1
1
1
1
1
1
Origin
Mouse
3
3
Source
Insect Cells
6
Protein Type
Recombinant
Purification tag / Conjugate
This IGSF9B protein is labelled with rho-1D4 tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
Sequence LVAQGAHGLR EEPEFVTARA GEGVVLRCDV IHPVTGQPPP YVVEWFKFGV PIPIFIKFGY YPPHVDPEYA GRASLHDKAS LRLEQVRSED QGWYECKVLM LDQQYDTFHN GSWVHLTINA PPTFTETPPQ YIEAKEGGSI TMTCTAFGNP KPIVTWLKEG TLLGASAKYQ VSDGSLTVTS VSREDRGAYT CRAYSIQGEA VHTTHLLVQG PPFIVSPPEN ITVNISQDAL LTCRAEAYPG NLTYTWYWQD ENVYFQNDLK LRVRILIDGT LIIFRVKPED AGKYTCVPSN SLGRSPSASA YLTVQYPARV LNMPPVIYVP VGIHGYIRCP VDAEPPATVV KWNKDGRPLQ VEKNLGWTLM EDGSIRIEEA TEEALGTYTC VPYNTLGTMG QSAPARLVLK DPPYFTVLPG WEYRQEAGRE LLIPCAAAGD PFPVITWRKV GKPSRSKHNA LPSGSLQFRA LSKEDHGEWE CVATNVVTSI TASTHLTVIG TSPHAPGSVR VHVSMTTANV SWEPGYDGGY EQTFSVWMKR AQFGPHDWLS LSVPPGPSWL LVDSLEPETA YQFSVLAQNR LGTSAFSEVV TVNTLAFPVT TPEPLVLVTP PRCLTANRTQ QGVLLSWLPP ANHSFPIDRY IMEFRVGERW EMLDDAIPGT DGDFFAKDLS QDTWYEFRVL AVMQDLISEP SNIAGVSSTD IFPQPDLTDD GLARPVLAGI VATICFLAAA ILFSTLAACF VNKQRKRKLK RKKDPPLSIT HCRKSLESPL SSGKVSPESI RTLRAPSESS DDQGQPAAKR MLSPTREKEL SLYKKTKRAI SSRKYSVAKA EAEAEATTPI ELISRGPDGR FVMGPSEMEP SVKGRRIEGF PFAEETDMYP EFRQSDEENE DPLVPTSVAA LKPQLTPMSS SQDSYLPPPA YSPRFQPRGL EGPSGLGGRL QATGQARPPA PRPFQHGQYY GYLSSSSPGE VEPPPFYMPE VGSPLSSVMS SPPLHTEGPF GHPTIPEENG ENASNSTLPL TQTPTGGRSP EPWGRPEFPF GGLETPAMMF PHQLHPCDVA ESLQPKACLP RGLPPAPLQV PAAYPGMLSL EAPKGWVGKS PGRGPIPAPP ATKWQERPMQ PLVSQGQLRH TSQGMGIPVL PYPEPAEPGG HGGPSTFGLD TRWYEPQPRP RPSPRQARRA EPSLHQVVLQ PSRLSPLTQS PLSSRTGSPE LAARARPRPG LLQQAEMSEI TLQPPAAVSF SRKSTPSSTG SPSQSSRSGS PSYRPTMGFT TLATGYPSPP PGPAPPAPGD TLDVFGQTPS PRRMGEEPLR PEPPTTLPTS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Igsf9b Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
  1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
  2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
  3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin-free.
Grade Crystallography grade
Protein Name
Background Transmembrane protein which is abundantly expressed in interneurons, where it may regulate inhibitory synapse development (By similarity). May mediate homophilic cell adhesion (PubMed:23751499). {ECO:0000250|UniProtKB:D3ZB51, ECO:0000269|PubMed:23751499}.
Molecular Weight 144.2 kD. Including tag.
UniProt E9PZ19
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Comment

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Images
Image no. 1 for Immunoglobulin Superfamily, Member 9B (IGSF9B) (AA 18-1328) protein (rho-1D4 tag) (ABIN3147301) „Crystallography Grade“ protein due to multi-step, protein-specific purification process