Histocompatibility 2, Class II Antigen E Alpha, Pseudogene (H2-EA-PS) (AA 26-255) protein (rho-1D4 tag)
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- Target See all Histocompatibility 2, Class II Antigen E Alpha, Pseudogene (H2-EA-PS) products
- Histocompatibility 2, Class II Antigen E Alpha, Pseudogene (H2-EA-PS)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 26-255
- Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- rho-1D4 tag
- Application
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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IKEEHTIIQA EFYLLPDKRG EFMFDFDGDE IFHVDIEKSE TIWRLEEFAK FASFEAQGAL ANIAVDKANL DVMKERSNNT PDANVAPEVT VLSRSPVNLG EPNILICFID KFSPPVVNVT WLRNGRPVTE GVSETVFLPR DDHLFRKFHY LTFLPSTDDF YDCEVDHWGL EEPLRKTWEF EEKTLLPETK ENVMCALGLF VGLVGIVVGI ILIMKGIKKR NVVERRQGAL GSSGTETSQV APA
Sequence includes N-terminal Rho1D4 tag - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human PLP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
- The protein is purified from the cleared cell lysate using Rho1D4-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin-free.
- Grade
- Crystallography grade
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Histocompatibility 2, Class II Antigen E Alpha, Pseudogene (H2-EA-PS) (AA 26-217) protein (His tag)
Crystallography grade H2-EA-PS Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Histocompatibility 2, Class II Antigen E Alpha, Pseudogene (H2-EA-PS) (AA 26-216) protein (His tag)Crystallography grade H2-EA-PS Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- In solution (20 mM Hepes, pH 7.4, 100 mM NaCl, 0,1 % Foscholine 12)
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- Histocompatibility 2, Class II Antigen E Alpha, Pseudogene (H2-EA-PS)
- Alternative Name
- H2-Ea (H2-EA-PS Products)
- Synonyms
- AI323765 Protein, E-alpha-f Protein, H-2Ea Protein, H2-Ea Protein, I-Ealpha Protein, Ia-3 Protein, Ia3 Protein, histocompatibility 2, class II antigen E alpha, pseudogene Protein, H2-Ea-ps Protein
- Molecular Weight
- 27.5 kDa including tag
- UniProt
- P01904
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