Band 3/AE1 Protein (AA 1-929) (Strep Tag)

Catalog No. ABIN3132502

Product Details

Target: Band 3/AE1 (SLC4A1) (Solute Carrier Family 4, Anion Exchanger, Member 1 (erythrocyte Membrane Protein Band 3, Diego Blood Group) (SLC4A1))

Protein Type: Recombinant

Protein Characteristics: AA 1-929

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This Band 3/AE1 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MGDMRDHEEV LEIPDRDSEE ELENIIGQIA YRDLTIPVTE MQDPEALPTE QTATDYVPSS TSTPHPSSGQ VYVELQELMM DQRNQELQWV EAAHWIGLEE NLREDGVWGR PHLSYLTFWS LLELQKVFSK GTFLLGLAET SLAGVANHLL DCFIYEDQIR PQDREELLRA LLLKRSHAED LGNLEGVKPA VLTRSGGASE PLLPHQPSLE TQLYCGQAEG GSEGPSTSGT LKIPPDSETT LVLVGRANFL EKPVLGFVRL KEAVPLEDLV LPEPVGFLLV LLGPEAPHVD YTQLGRAAAT LMTERVFRIT ASMAHNREEL LRSLESFLDC SLVLPPTDAP SEKALLNLVP VQKELLRRRY LPSPAKPDPN LYNTLDLNGG KGGPGDEDDP LRRTGRIFGG LIRDIRRRYP YYLSDITDAL SPQVLAAVIF IYFAALSPAV TFGGLLGEKT RNLMGVSELL ISTAVQGILF ALLGAQPLLV LGFSGPLLVF EEAFFSFCES NNLEYIVGRA WIGFWLILLV MLVVAFEGSF LVQYISRYTQ EIFSFLISLI FIYETFSKLI KIFQDYPLQQ TYAPVVMKPK PQGPVPNTAL FSLVLMAGTF LLAMTLRKFK NSTYFPGKLR RVIGDFGVPI SILIMVLVDS FIKGTYTQKL SVPDGLKVSN SSARGWVIHP LGLYRLFPTW MMFASVLPAL LVFILIFLES QITTLIVSKP ERKMIKGSGF HLDLLLVVGM GGVAALFGMP WLSATTVRSV THANALTVMG KASGPGAAAQ IQEVKEQRIS GLLVSVLVGL SILMEPILSR IPLAVLFGIF LYMGVTSLSG IQLFDRILLL FKPPKYHPDV PFVKRVKTWR MHLFTGIQII CLAVLWVVKS TPASLALPFV LILTVPLRRL ILPLIFRELE LQCLDGDDAK VTFDEENGLD EYDEVPMPV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Band 3/AE1 (SLC4A1) (Solute Carrier Family 4, Anion Exchanger, Member 1 (erythrocyte Membrane Protein Band 3, Diego Blood Group) (SLC4A1))

Alternative Name: Slc4a1 (SLC4A1 Products)

Synonyms: AE1 Protein, BND3 Protein, CD233 Protein, DI Protein, EMPB3 Protein, EPB3 Protein, FR Protein, RTA1A Protein, SW Protein, WD Protein, WD1 Protein, WR Protein, Ae1 Protein, Empb3 Protein, l11Jus51 Protein, ae1 Protein, band3 Protein, MGC152771 Protein, zgc:111889 Protein, zgc:152771 Protein, si:dz180g5.1 Protein, LOC100136769 Protein, slc4a1 Protein, wd1 Protein, bnd3 Protein, epb3 Protein, cd233 Protein, empb3 Protein, rta1a Protein, MGC80391 Protein, SLC4A1 Protein, BB3 Protein, EAT Protein, solute carrier family 4 member 1 (Diego blood group) Protein, solute carrier family 4 (anion exchanger), member 1 Protein, solute carrier family 4 (anion exchanger), member 1a (Diego blood group) Protein, Band 3 Protein, erythrocyte membrane protein band 4.1 like 3 Protein, solute carrier family 4 member 1 (Diego blood group) L homeolog Protein, solute carrier family 4 member 1 Protein, SLC4A1 Protein, Slc4a1 Protein, slc4a1a Protein, LOC100136769 Protein, EPB41L3 Protein, slc4a1.L Protein, slc4a1 Protein

Background: Band 3 anion transport protein (Anion exchange protein 1) (AE 1) (Anion exchanger 1) (MEB3) (Solute carrier family 4 member 1) (CD antigen CD233),FUNCTION: Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Component of the ankyrin-1 complex of the erythrocyte membrane, required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine. {ECO:0000250|UniProtKB:P02730}.

Molecular Weight: 103.1 kDa

UniProt: P04919