OPN1SW Protein (AA 1-346) (Strep Tag)

Catalog No. ABIN3134356

Product Details

Target: OPN1SW (Opsin 1 (Cone Pigments), Short-Wave-Sensitive (OPN1SW))

Protein Type: Recombinant

Protein Characteristics: AA 1-346

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This OPN1SW protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MSGEDDFYLF QNISSVGPWD GPQYHLAPVW AFRLQAAFMG FVFFVGTPLN AIVLVATLHY KKLRQPLNYI LVNVSLGGFL FCIFSVFTVF IASCHGYFLF GRHVCALEAF LGSVAGLVTG WSLAFLAFER YVVICKPFGS IRFNSKHALM VVLATWIIGI GVSIPPFFGW SRFIPEGLQC SCGPDWYTVG TKYRSEYYTW FLFIFCFIIP LSLICFSYSQ LLRTLRAVAA QQQESATTQK AEREVSHMVV VMVGSFCLCY VPYAALAMYM VNNRNHGLDL RLVTIPAFFS KSSCVYNPII YCFMNKQFRA CILEMVCRKP MADESDVSGS QKTEVSTVSS SKVGPH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: OPN1SW (Opsin 1 (Cone Pigments), Short-Wave-Sensitive (OPN1SW))

Alternative Name: Opn1sw (OPN1SW Products)

Synonyms: SWS2 Protein, bluops Protein, zfblue Protein, SI:zK13A21.5 (blue-sensitive opsin) Protein, Blop Protein, GB13493 Protein, SWS1 Protein, bcp Protein, bop Protein, cbt Protein, CHK-2 Protein, BCP Protein, BOP Protein, CBT Protein, AW551857 Protein, Bcp Protein, opsin 1 (cone pigments), short-wave-sensitive 2 Protein, opsin 1, short wave sensitive Protein, blue-sensitive opsin Protein, KFH-B protein Protein, opsin 1 (cone pigments), short-wave-sensitive Protein, blue sensitive cone opsin Protein, opsin 1 (cone pigments), short-wave-sensitive (color blindness, tritan) Protein, opn1sw2 Protein, OPN1SW Protein, Blop Protein, kfh-b Protein, opn1sw Protein, Opn1sw Protein, OPN2SW Protein

Background: Short-wave-sensitive opsin 1 (S opsin) (Blue cone photoreceptor pigment) (Blue-sensitive opsin) (BOP) (Short wavelength-sensitive cone opsin),FUNCTION: Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal (By similarity). Required for the maintenance of cone outer segment organization in the ventral retina, but not essential for the maintenance of functioning cone photoreceptors (PubMed:21219924, PubMed:25416279). Involved in ensuring correct abundance and localization of retinal membrane proteins (PubMed:25416279). May increase spectral sensitivity in dim light (PubMed:11055434). {ECO:0000250|UniProtKB:P03999, ECO:0000269|PubMed:11055434, ECO:0000269|PubMed:21219924, ECO:0000269|PubMed:25416279}.

Molecular Weight: 38.9 kDa

UniProt: P51491