SREBF2 Protein (AA 1-1130) (Strep Tag)

Catalog No. ABIN3135009

Product Details

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Protein Type: Recombinant

Protein Characteristics: AA 1-1130

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SREBF2 protein is labelled with Strep Tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequence: MDESSELGVL ETMETLTELG DELTLGDIDE MLQFVSNQVG EFPDLFSEQL CSSFPGGGSN GGSGNNSSGR GNNGGATDPA VQRSFSQVPL STFSPSAASP QAPALQVKVS PTPPRATPVL QPRPQPQPQP PAQLQQQTVM ITPTFSTAPQ TRIIQQPLIY QNAATSFQVL QPQVQSLVTS PQVQPVTIQQ QVQTVQAQRV LTQTANGTLQ TLAPATVQTV AAPQVQQVPV LVQPQIIKTD SLVLTTLKTD GSPVMAAVQN PALTALTAPI QTAALQVPTL VGSNGTILTT MPVMMGQEKV PIKQVPGGVK QLDPPKEGER RTTHNIIEKR YRSSINDKII ELKDLVMGTD AKMHKSGVLR KAIDYIKYLQ QVNHKLRQEN MVLKLANQKN KLLKGIDLGS LVDSDVDLKI DDFNQNVLLM SPPASDSGSQ AGFSPYSIDS EPGSPLLDDA KVKDEPDSPP VALGMVDRSR ILLCVLTFLG LSFNPLTSLL QWGGAHNTDQ HPYSGSGRSV LSLESGAGGW FDWMVPTLLL WLVNGVIVLS VFVKLLVHGE PVIRPHSRPS VTFWRHRKQA DLDLAKGDFA AAAANLQTCL SVLGRALPTS RLDLACSLSW NVIRYSLQKL RLVRWLLKKV FQRWRATPAT AAGFEDEAKS SARDAALAYH RLHQLHITGK LPAGSACSDV HMALCAVNLA ECAEEKILPS TLIEIHLTAA MGLKTRCGGK LGFLASYFLN RAQSLCGPEH STVPDSLRWL CHPLGQKFFM ERSWSIKSAA KESLYCAQRS PADPIAQVHQ AFCKNLLERA VESLVKPQAK KKAGDQEEES CEFSSALEYL KLLHSFVDSV GFVTSPFSSS SVLRSALGPD VICRWWTSAV TMAISWLQGD DAAVRSRFTE VERVPKALEV TESPLVKAVF YTCRAMHASL SGKADGQQNS FCHCERASGH LWSSLNVSGT TSDPSLNHVI QLFTCDLLLS LRTALWQKQA SASQLLGETY HASGTELAGF QRDLGSLRRL AHSFRPAYRK VFLHEATVRL MAGASPTRTH QLLEHSLRRR PTQNTKHGEV DTWPGQRERA TAILLACRHL PLSFLSSPGQ RAVLLAEAAR TLEKVGDRRS CSDCQQMIVK LGGGTAIAAS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Alternative Name: Srebf2 (SREBF2 Products)

Synonyms: SREBF2 Protein, srebp2 Protein, SREBP2 Protein, zgc:158371 Protein, AI608257 Protein, SREBP-2 Protein, SREBP2gc Protein, bHLHd2 Protein, lop13 Protein, nuc Protein, Srebf2_retired Protein, sterol regulatory element binding transcription factor 2 Protein, sterol regulatory element binding factor 2 Protein, sterol regulatory element binding transcription factor 2 L homeolog Protein, SREBF2 Protein, srebf2 Protein, Srebf2 Protein, srebf2.L Protein

Background: Sterol regulatory element-binding protein 2 (SREBP-2) (Sterol regulatory element-binding transcription factor 2) [Cleaved into: Processed sterol regulatory element-binding protein 2 (Transcription factor SREBF2)],FUNCTION: [Sterol regulatory element-binding protein 2]: Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 2), which is embedded in the endoplasmic reticulum membrane (By similarity). Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis (PubMed:9616204, PubMed:16100574). {ECO:0000250|UniProtKB:Q12772, ECO:0000269|PubMed:16100574, ECO:0000269|PubMed:9616204}., FUNCTION: [Processed sterol regulatory element-binding protein 2]: Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis (PubMed:9616204). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3') (By similarity). Regulates transcription of genes related to cholesterol synthesis pathway (PubMed:9616204). {ECO:0000250|UniProtKB:Q12772, ECO:0000269|PubMed:9616204}.

Molecular Weight: 122.9 kDa

UniProt: Q3U1N2

Pathways: Regulation of Lipid Metabolism by PPARalpha