SLC4A10 Protein (AA 1-1118) (Strep Tag)

Catalog No. ABIN3152040

Product Details

Target: SLC4A10 (Solute Carrier Family 4, Sodium Bicarbonate Transporter, Member 10 (SLC4A10))

Protein Type: Recombinant

Protein Characteristics: AA 1-1118

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SLC4A10 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MEIKDQGAQM EPLLPTRNDE EAVVDRGGTR SILKTHFEKE DLEGHRTLFI GVHVPLGGRK SHRRHRHRGH KHRKRDRERD SGLEDGRESP SFDTPSQRVQ FILGTEDDDE EHLPHDLFTE LDEICWREGE DAEWRETARW LKFEEDVEDG GERWSKPYVA TLSLHSLFEL RSCILNGTVL LDMHANTIEE IADMVLDQQV SSGQLNEDVR HRVHEALMKQ HHHQNQKKLA NRIPIVRSFA DIGKKQSEPN SMDKNAGQVV SPQSAPACAE NKNDVSRENS TVDFSKGLGG QQKGHTSPCG MKQRLDKGPP HQQEREVDLH FMKKIPPGAE ASNILVGELE FLDRTVVAFV RLSPAVLLQG LAEVPIPSRF LFILLGPLGK GQQYHEIGRS IATLMTDEVF HDVAYKAKDR NDLVSGIDEF LDQVTVLPPG EWDPSIRIEP PKNVPSQEKR KIPAVPNGTA AHGEAEPHGG HSGPELQRTG RIFGGLILDI KRKAPFFWSD FRDAFSLQCL ASFLFLYCAC MSPVITFGGL LGEATEGRIS AIESLFGASM TGIAYSLFGG QPLTILGSTG PVLVFEKILF KFCKEYGLSY LSLRASIGLW TATLCIILVA TDASSLVCYI TRFTEEAFAS LICIIFIYEA LEKLFELSET YPINMHNDLE LLTQYSCNCM EPHSPSNDTL KEWRESNLSA SDIIWGNLTV SECRSLHGEY VGRACGHGHP YVPDVLFWSV ILFFSTVTMS ATLKQFKTSR YFPTKVRSIV SDFAVFLTIL CMVLIDYAIG IPSPKLQVPS VFKPTRDDRG WFVTPLGPNP WWTIIAAIIP ALLCTILIFM DQQITAVIIN RKEHKLKKGC GYHLDLLMVA VMLGVCSIMG LPWFVAATVL SITHVNSLKL ESECSAPGEQ PKFLGIREQR VTGLMIFILM GSSVFMTSIL KFIPMPVLYG VFLYMGASSL KGIQLFDRIK LFWMPAKHQP DFIYLRHVPL RKVHLFTVIQ MSCLGLLWII KVSRAAIVFP MMVLALVFVR KLMDFLFTKR ELSWLDDLMP ESKKKKLEDA EKEEEQSMLA MEDEGTVQLP LEGHYRDDPS VINISDEMSK TAMWGNLLVT ADNSKEKESR FPSKSSPS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SLC4A10 (Solute Carrier Family 4, Sodium Bicarbonate Transporter, Member 10 (SLC4A10))

Alternative Name: Slc4a10 (SLC4A10 Products)

Synonyms: ncbe Protein, nbcn2 Protein, NCBE Protein, mKIAA4136 Protein, NBCn2 Protein, solute carrier family 4 member 10 Protein, solute carrier family 4, sodium bicarbonate transporter, member 10 Protein, solute carrier family 4, sodium bicarbonate transporter, member 10 L homeolog Protein, solute carrier family 4, sodium bicarbonate cotransporter-like, member 10 Protein, SLC4A10 Protein, slc4a10 Protein, slc4a10.L Protein, Slc4a10 Protein

Background: Sodium-driven chloride bicarbonate exchanger (Solute carrier family 4 member 10),FUNCTION: Sodium/bicarbonate cotransporter which plays an important role in regulating intracellular pH (PubMed:20566632). Has been shown to act as a sodium/bicarbonate cotransporter in exchange for intracellular chloride (PubMed:20566632). Has also been shown to act as a sodium/biocarbonate cotransporter which is not responsible for net efflux of chloride, with the observed chloride efflux being due to chloride self-exchange (By similarity). Controls neuronal pH and may contribute to the secretion of cerebrospinal fluid (PubMed:18165320). Reduces the excitability of CA1 pyramidal neurons and modulates short-term synaptic plasticity (PubMed:26136660). Required in retinal cells to maintain normal pH which is necessary for normal vision (PubMed:23056253). In the kidney, likely to mediate bicarbonate reclamation in the apical membrane of the proximal tubules (By similarity). {ECO:0000250|UniProtKB:Q6U841, ECO:0000250|UniProtKB:Q80ZA5, ECO:0000269|PubMed:18165320, ECO:0000269|PubMed:20566632, ECO:0000269|PubMed:23056253, ECO:0000269|PubMed:26136660}., FUNCTION: [Isoform 2]: Sodium/bicarbonate cotransporter which mediates cotransport of sodium and bicarbonate in association with an efflux of intracellular chloride. {ECO:0000269|PubMed:10993873}.

Molecular Weight: 125.8 kDa

UniProt: Q5DTL9