ATP13A4 Protein (AA 1-1193) (Strep Tag)
Crystallography grade
ATP13A4
Origin: Mouse
Host: Tobacco (Nicotiana tabacum)
Recombinant
≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, SDS, WB
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- Target See all ATP13A4 products
- ATP13A4 (ATPase Type 13A4 (ATP13A4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1193
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Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This ATP13A4 protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MGDHLEKSQH ALLNEGDENE MEIFGYRTQG CRKALCLIGS IFSLGMLPLV FYWRPAWRVW ANCVPCSLQE ADVVLLKTTD EFKIYSWKKV IWISLSALSS TSGLTPDHPL ITDEGYIINR AIRKPDLKVR YIKVQKIRYV WNNLEGQFQK IGSLEDWLSS AKIHQKFGLG LTSEEQEIRR LICGPNAIDV EITPIWKLLI KEVLNPFYIF QLFSVCLWFS EDYKEYALAI ILMSVISIAL TVYDLRQQSV KLHHLVESHN SITVSVYERK AGAQDLESRL LVPGDLLILT GSRVQMPCDA ILIDGSCVVD EGMLTGESIP VTKTPLSQTA SSVPWKMQSE ADPRRHVLFC GTEVIQAKAA GSGAVRAVVL QTGFNTAKGD LVRSILYPKP MNFKLYRDAI RFLLCLVGTA TIGMVYTLCV YVLSGEPPEE VVRKALDVIT IAVPPALPAA LTTGIIYAQR RLKKKGIFCI SPQRINVCGQ LNLVCFDKTG TLTRGGLDPW GVVPCDQNGF QAVHSFASGK ALPQGPLCAA MASCHSLILL DGTIQGDPLD LKMFEATKWE MTASGDDLHI KEMLAHTIVV KPTDMVGQVP AEGLAIVHQF PFSSALQRMT VIVQEMGGGR LAFMKGAPER VASFCQPDTV PTSFISELQI YTTQGFRVIA LAYKKLEMDC PTTALMREKV ESDLVFLGLL ILENRLKEET KPVLEELISA RIRTVMITGD NLQTAITVAR KSGMVSEGQK VILVEANEAT GSSSASISWK LVEEKKPGPF GSQDTYINIR EEVPENGRDR SYHFALSGKS FHVISQYFSS LLPKILINGT IFARMSPGQK SSLVEEFQKL DYFVGMCGDG ANDCGALKMA HVGISLSEQE ASVASPFTSK TPNIECVPHL IKEGRAALVT SFCMFKYMAL YSMIQYVGVL LLYWKTNSLS NYQFLFQDLA ITTLIGVTMN LNGANPKLVP FRPAGRLISP PLLLSVVLNI LLSLAMHIVG FILVQKQPWY IMDYHSVCPV RNESASALAA SPSVPEKTRS NSTFASFENT TIWFLGTINC IFVALVFSKG KPFRQPTYTN YIFVLVLILQ MGVCLFILFA DIPEMHRRLD LLCTPVLWRV YILIMISSNF VVSLAVEKAI IENRALWIAV KRCFGYQSKS QYRIWQRNLA NDSSWPPLNQ TSYSDMQGVS YSNPVFESNE EQL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- ATP13A4 (ATPase Type 13A4 (ATP13A4))
- Alternative Name
- Atp13a4 (ATP13A4 Products)
- Synonyms
- 4631413J11Rik Protein, 4832416L12 Protein, 9330174J19Rik Protein, ATPase 13A4 Protein, ATPase type 13A4 Protein, probable cation-transporting ATPase 13A4 Protein, ATP13A4 Protein, LOC100013946 Protein, Atp13a4 Protein
- Background
- Probable cation-transporting ATPase 13A4 (EC 7.2.2.-) (P5-ATPase isoform 4)
- Molecular Weight
- 132.8 kDa
- UniProt
- Q5XF90
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