ABCA8A Protein (AA 1-1620) (Strep Tag)
Crystallography grade
ABCA8A
Origin: Mouse
Host: Tobacco (Nicotiana tabacum)
Recombinant
≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, SDS, WB
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- Target See all ABCA8A products
- ABCA8A (ATP-Binding Cassette, Sub-Family A (ABC1), Member 8a (ABCA8A))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1620
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This ABCA8A protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MVKREINVCQ QTWALLCKNL LRKKRLKRDT FLEFLYTALI LLSLILFLQL HEVYDFSSLP DVDLGRIDSF NDSTFMIVYT PITPTTQRIM DRVSLVSYMT GRKILASPNE ENMTELISMR FSDVVGVIFT NAYSYNLKFI KGARIPTIKE HQDHTAHCHS YGEIIYCGLS EFWRDGFVAL QAAINAAIIE VTTNHSVMEE MMSLTGKYIK IDSFVGQEGT TTDCFLFFCI IRFSPLTYYI SAGVTRERKK MKGLMAVMGL RDSAFWLSWG LLYGVIVFVV TLLSTTIVKL VQFVFLTGFM VIFSLFFFYG LSLISLSFLM SVLLKKSFLT DLVVFLLTVS CGSLGFTALY RYLPVSLEWL LSLLSPFAFM LGMVQLLRLD YDVNSNADPM GNPNEVIGTI FMLFFDGVFY LLLTFYFEKV LPSKSFHDKT YWHACKSHFF LIDYSFYIRT ALDNETDYEF SDDSFEPVSM EFHGKEAIRI RNLTKDYIQK SKRTEALKDL TLDVYKGQIT AILGHSGAGK STLLNVLSGL CVPTKGWVTI HNNKLSEMTD LENISKLTGV CPQCNVQFDF LTVRENLRLF AKIKGIQAHE VDNEVQRVLL ELDMKNTQNI LVQNLSGGQK RKLTFGIAIL GDPQIFLLDE PTAGLDPFSR HRVWNFLKER RADRVVLFST QFMDEADILA DRKVFISKGK LKCAGSSLFL KKKWGIGYHL SLQLSETCVH ERITSLVKQH IPDSKLSAES EGKLSYILPL ERTNKFPDLY RDLERSPDLG IENYGVSITT LTEVFLKLEG KSSIDQSDIG MTEDVQAGGA RSPERFAEVE QLVSLLNGRC KMKGGMALWW QQLCAVTRLR FLKLKHERKS IVILILVLGI GLLHILSANI YRMVRQSDYC WELAPHMYFL TPGQQPQPPL TNLLIVNKTG AKIDDFIHSL EQQNIALEVD AFGTRNGTED SQYNGAIILS GDEKNYNFTL ACNTKRLNCF PVLVDIVSNG LLGLFAPSAH IQTDRSTFPE ENDHRKFDYL AYFFLWVLLM ACVPPYISMT SIDDYKNRAQ FQLWISGLSP SAYWFGQALF EVPVYCALIL SIFIAFYASA PPESKFTVGD LFIQILYVGG YAMSVIFMTY VISFIYRKGR KNSGLWSLCF YIVSFFSMCF MLIDYFRDIS LFVLIALVPP ATLGGCTLLH FENREFSEII FEPEREYSYL FFLAPLLHFA IFVVILRCME RKFGMKTMRT DPVFRISPRS DRVFNNPEDP DGEDEDVSQE RVWTANALTS ADFQEKPAII ASCLRKEYKG KKKCFVLKSK KKIATRNISF CVRKGEVVGL LGHNGAGKST SIKMITGETK PSAGQVLLKG SSTGDTPGFL GYCPQENALW LNLTVREHLE IFAAIKGMRK SDANVAIERL ADALKLQDQL KSPVKTLSEG VKRKLCFVLS ILGNPSVVLL DEPSTGMDPE GQQQMWQAIQ ATFSNTERGA LLTTHYMAEA EAVCDRVAIM VSGRLRCIGS IQHLKSKFGK EYLLEMKVKT PSQVEPLNTE IMRLFPQAAR QERYSSLMVY KLPREDVQPL SQAFFKLETV KQSFDLEEYS LSQSTLEQVF LELSKEQELD GFEEELDPSV KWKLLPQEEA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- ABCA8A (ATP-Binding Cassette, Sub-Family A (ABC1), Member 8a (ABCA8A))
- Alternative Name
- Abca8a (ABCA8A Products)
- Synonyms
- ATP-binding cassette, sub-family A (ABC1), member 8a Protein, Abca8a Protein
- Background
- ABC-type organic anion transporter ABCA8A (EC 7.6.2.-) (ATP-binding cassette sub-family A member 8A),FUNCTION: Mediates cholesterol and taurocholate efflux. Through the interaction with ABCA1 potentiates the cholesterol efflux to lipid-free APOA1, in turn regulates high-density lipoprotein cholesterol levels. {ECO:0000250|UniProtKB:O94911}.
- Molecular Weight
- 184.2 kDa
- UniProt
- Q8K442
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