ABCA5 Protein (AA 1-1642) (Strep Tag)

Catalog No. ABIN3136599

Product Details

Target: ABCA5 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 5 (ABCA5))

Protein Type: Recombinant

Protein Characteristics: AA 1-1642

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This ABCA5 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MATAIRDVGV WRQTRTLLLK NYLIKCRTKK SSVQEILFPL FFLFWLILVS MMHPNKKYEE VSDIELSPMD KFSLSNVILG YTPVTNITSS IMQRVSTDHL PKVIVTEEYA NEKELVAASL SKSSNFVGVV FKDTMSYELR FFPEMIPVSS IYMNSREGCS KTCDAAQYWS LGFTVLQASI DAAIIQLKTN VSVWSELEST KAVIMGEAAV VEIDTFPRGV ILIYLVIAFS PFGYFLAIHI VAEKEKKLKE FLKIMGLHDT AFWLSWVLLY ASLIFLMSLL MAVIATASSL FPQSSSIVIF LLFFLYGLSS VFFALMLTPL FKKSKHVGVV EFFVTVVFGF VGLLIVLIES FPRSLVWLFS PLCQCAFLIG IAQVMHLEDF NEGALFSNLT EGPYPLIITI IMLALDSVFY VLLAVYLDQV IPGEFGLRRS SLYFLKPSYW SKNKRNYKEL SEGNINGNIS LNEIVEPVSS EFIGKEAIRI SGIQKSYRKK TENVEALRNL SFDIYEGQIT ALLGHSGTGK STLMNILCGL CPPSDGFASI YGHRVSEIDE MFEARKMIGI CPQSDINFDV LTVEENLSIL ASIKGIPANN IIQEVQKVLL DLDMQAIKDN QAKKLSGGQK RKLSVGIAVL GNPKILLLDE PTAGMDPCSR HIVWNLLKYR KANRVTVFST HFMDEADILA DRKAVISQGM LKCVGSSIFL KSKWGIGYRL SMYIDRYCAT ESLSSLVRQH IPAAALLQQN DQQLVYSLPF KDMDKFSGLF SALDIHSNLG VISYGVSMTT LEDVFLKLEV EAEIDQADYS VFTQQPREEE TDSKSFDEME QSLLILSETK ASSVSTMSLW KQQVSTIAKF HFLSLKRESK SVRAVLLLLL IFFAVQIFMF FLHHSFKNAV VPIKLVPDLY FLKPGDKPHK YKTSLLLQNS TDSDINGLIE FFAHQNIMVA MFNDSDYVSA APHSAALNVV RSEKDYVFSA VFNSTMVYCL PVMMNIISNY YLYHLNVTEA IQTWSTPFIQ EITDIVFKIE LYFQAALLGI IVTAMPPYFA MENAENHKIK AYTQLKLSGL LPSAYWVGQA VVDIPLFFVV LILMLGSLFA FHHGLYFYPA KFLAVVFCLI AYVPSVILFT YIASFTFKKI LNTKEFWSFI YSVTALACVA ITETTFFLQY AVTAVFHYTF CIAIPIYPLL GCLISFIKGS WKNMPKNENT YNPWDRLLVA VIMPYLQCIL WIFLLQHYEK IHGGRSIRKD PFFRALSQKA KNKKFPEPPI NEDEDEDVKA ERLKVKELMG CQCCEEKPAI MVCNLHKEYD DKKDFLHSRK TTKVATKYIS FCVKKGEILG LLGPNGAGKS TVINTLVGDV EPTSGKIFLG DYGSHSSEDD ESIKCMGYCP QTNPLWPDLT LQEHFEIYGA VKGMSPGDMK EVISRITKAL DLKEHLQKTV KKLPAGIKRK LCFALSMLGN PQVTLLDEPS TGMDPRAKQH MWRAIRTAFK NKKRAALLTT HYMEEAEAVC DRVAIMVSGQ LRCIGTVQHL KSKFGKGYFL EIKLKDWIEN LEIDRLQREI QYIFPNASRQ ESFSSILAFK IPKEDVQSLS QSFAKLEEAK RTFAIEEYSF SQATLEQVFV ELTKEQEEED NSCGTLASTL WWERTQEDRV VF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ABCA5 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 5 (ABCA5))

Alternative Name: Abca5 (ABCA5 Products)

Synonyms: zgc:163009 Protein, ABC13 Protein, EST90625 Protein, B930033A02Rik Protein, mKIAA1888 Protein, ATP binding cassette subfamily A member 5 Protein, ATP-binding cassette, sub-family A (ABC1), member 5 Protein, ATP-binding cassette sub-family A member 5 Protein, ABCA5 Protein, abca5 Protein, LOC100545445 Protein, Abca5 Protein

Background: Cholesterol transporter ABCA5 (EC 7.6.2.-) (ATP-binding cassette sub-family A member 5),FUNCTION: Cholesterol efflux transporter in macrophages that is responsible for APOAI/high-density lipoproteins (HDL) formation at the plasma membrane under high cholesterol levels and participates in reverse cholesterol transport (PubMed:25125465, PubMed:32687853, PubMed:20382126). May play a role in the processing of autolysosomes (PubMed:15870284). {ECO:0000269|PubMed:15870284, ECO:0000269|PubMed:20382126, ECO:0000269|PubMed:25125465, ECO:0000269|PubMed:32687853}.

Molecular Weight: 185.9 kDa

UniProt: Q8K448