GLCE Protein (AA 1-618) (Strep Tag)

Catalog No. ABIN3129755

Product Details

Target: GLCE (Glucuronic Acid Epimerase (GLCE))

Protein Type: Recombinant

Protein Characteristics: AA 1-618

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This GLCE protein is labelled with Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Sequence: MRCLAARVNY KTLIIICALF TLVTVLLWNK CSSDKAIQFP RHLSSGFRVD GLEKRSAASE SNHYANHIAK QQSEEAFPQE QQKAPPVVGG FNSNGGSKVL GLKYEEIDCL INDEHTIKGR REGNEVFLPF TWVEKYFDVY GKVVQYDGYD RFEFSHSYSK VYAQRSPYHP DGVFMSFEGY NVEVRDRVKC ISGVEGVPLS TQWGPQGYFY PIQIAQYGLS HYSKNLTEKP PHIEVYETAE DRDRNIRPNE WTVPKGCFMA SVADKSRSTN VKQFIAPETS EGVSLQLGNT KDFIISFDLK LLTNGSVSVV LETTEKNQLF TVHYVSNTQL IAFRDRDIYY GIGPRTSWST VTRDLVTDLR KGVGLSNTKA VKPTKIMPKK VVRLIAKGKG FLDNITISTT AHMAAFFAAS DWLVRNQDEK GGWPIMVTRK LGEGFKSLEP GWYSAMAQGQ AISTLVRAYL LTKDYVFLSS ALRATAPYKF PSEQHGVKAV FMNKHDWYEE YPTTPSSFVL NGFMYSLIGL YDLKETAGET LGKEARSLYE RGMESLKAML PLYDTGSGTI YDLRHFMLGI APNLARWDYH TTHINQLQLL STIDESPIFK EFVKRWKSYL KGSRAKHN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: GLCE (Glucuronic Acid Epimerase (GLCE))

Alternative Name: Glce (GLCE Products)

Synonyms: HSEPI Protein, RGD1565253 Protein, MGC79110 Protein, 1110017N23Rik Protein, AI747411 Protein, C130034A12Rik Protein, Hsepi Protein, glce Protein, glcel Protein, sb:eu394 Protein, zgc:136701 Protein, glucuronic acid epimerase Protein, glucuronic acid epimerase L homeolog Protein, glucuronyl C5-epimerase Protein, glucuronic acid epimerase b Protein, glucuronic acid epimerase a Protein, GLCE Protein, Glce Protein, glce.L Protein, glce Protein, glceb Protein, glcea Protein

Background: D-glucuronyl C5-epimerase (EC 5.1.3.17) (Heparan sulfate C5-epimerase) (Hsepi) (Heparin sulfate C5-epimerase) (Heparin/heparan sulfate:glucuronic acid C5-epimerase) (Heparosan-N-sulfate-glucuronate 5-epimerase),FUNCTION: Converts D-glucuronic acid residues adjacent to N-sulfate sugar residues to L-iduronic acid residues, both in maturing heparan sulfate (HS) and heparin chains. This is important for further modifications that determine the specificity of interactions between these glycosaminoglycans and proteins. {ECO:0000269|PubMed:11274177, ECO:0000269|PubMed:11279150, ECO:0000269|PubMed:16532012, ECO:0000269|PubMed:20208005}.

Molecular Weight: 70.1 kDa

UniProt: Q9EPS3

Pathways: Glycosaminoglycan Metabolic Process