KCNQ5 Protein (AA 1-933) (Strep Tag)

Catalog No. ABIN3137305

Product Details

Target: KCNQ5 (Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 5 (KCNQ5))

Protein Type: Recombinant

Protein Characteristics: AA 1-933

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This KCNQ5 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MPRHHAGGEE GGAAGLWVRS GAAAAAGAGG GRPGSGMKDV ESGRGRVLLN SAAARGDGLL LLGTRAAALG GGGGGLRESR RGKQGARMSL LGKPLSYTSS QSCRRNVKYR RVQNYLYNVL ERPRGWAFVY HAFVFLLVFG CLILSVFSTI PEHTKLASSC LLILEFVMIV VFGLEFIIRI WSAGCCCRYR GWQGRLRFAR KPFCVIDTIV LIASIAVVSA KTQGNIFATS ALRSLRFLQI LRMVRMDRRG GTWKLLGSVV YAHSKELITA WYIGFLVLIF SSFLVYLVEK DANKEFSTYA DALWWGTITL TTIGYGDKTP LTWLGRLLSA GFALLGISFF ALPAGILGSG FALKVQEQHR QKHFEKRRNP AANLIQCVWR SYAADEKSVS IATWKPHLKA LHTCSPTKKE QGEASSSQKL SFKERVRMAS PRGQSIKSRQ ASVGDRRSPS TDITAEGSPT KVQKSWSFND RTRFRPSLRL KSSQPKPVID ADTALGIDDV YDEKGCQCDV SVEDLTPPLK TVIRAIRIMK FHVAKRKFKE TLRPYDVKDV IEQYSAGHLD MLCRIKSLQT RVDQILGKGQ MTSDKKSREK ITAEHETTDD PSMLARVVKV EKQVQSIESK LDCLLDIYQQ VLRKGSASAL TLASFQIPPF ECEQTSDYQS PVDSKDLSGS AQNSGCLTRS ASANISRGLQ FILTPNEFSA QTFYALSPTM HSQATQVPMS QNDGSSVVAT NNIANQISAA PKPAAPTTLQ IPPPLSAIKH LSRPEPLLSN PTGLQESISD VTTCLVASKE SVQFAQSNLT KDRSLRKSFD MGGETLLSVR PMVPKDLGKS LSVQNLIRST EELNLQFSGS ESSGSRGSQD FYPKWRESKL FITDEEVGAE ETETDTFDGT PPPAGEAAFS SDSLRTGRSR SSQNICKTGD STDALSLPHV KLN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: KCNQ5 (Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 5 (KCNQ5))

Alternative Name: Kcnq5 (KCNQ5 Products)

Synonyms: KCNQ5 Protein, si:dkey-29l6.1 Protein, Kcnq5l Protein, Kv7.5 Protein, 7730402H11 Protein, 9230107O05Rik Protein, AA589396 Protein, D1Mgi1 Protein, potassium voltage-gated channel subfamily Q member 5 Protein, potassium voltage-gated channel, KQT-like subfamily, member 5a Protein, potassium voltage-gated channel, KQT-like subfamily, member 5 Protein, potassium voltage-gated channel, subfamily Q, member 5 Protein, KCNQ5 Protein, kcnq5a Protein, Kcnq5 Protein

Background: Potassium voltage-gated channel subfamily KQT member 5 (KQT-like 5) (Potassium channel subunit alpha KvLQT5) (Voltage-gated potassium channel subunit Kv7.5),FUNCTION: Associates with KCNQ3 to form a potassium channel which contributes to M-type current, a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons. Therefore, it is important in the regulation of neuronal excitability. May contribute, with other potassium channels, to the molecular diversity of a heterogeneous population of M-channels, varying in kinetic and pharmacological properties, which underlie this physiologically important current. {ECO:0000269|PubMed:15963599}.

Molecular Weight: 102.3 kDa

UniProt: Q9JK45