Hephaestin Protein (HEPH) (AA 19-1157) (rho-1D4 tag)

Catalog No. ABIN3137582

Product Details

Target: Hephaestin (HEPH)

Protein Type: Recombinant

Protein Characteristics: AA 19-1157

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This Hephaestin protein is labelled with rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: IPTDGAIRNY YLGIQDMQWN YAPKGRNVIT NQTLNNDTVA SSFLKSGKNR IGSSYKKTVY KEYSDGTYTE EIAKPAWLGF LGPLLQAEVG DVILIHLKNF ASRPYTIHPH GVFYEKDSEG SLYPDGSSGY LKADDSVPPG GSHVYNWSIP ESHAPTEADP ACLTWIYHSH VDAPRDIATG LIGPLITCKR GTLDGNSPPQ RKDVDHNFFL LFSVIDENLS WHLDDNIATY CSDPASVDKE DGAFQDSNRM HAINGFVFGN LPELSMCAQK HVAWHLFGMG NEIDVHTAFF HGQMLSIRGH HTDVANIFPA TFVTAEMVPQ KSGTWLISCE VNSHLRSGMQ AFYKVDSCSM DPPVDQLTGK VRQYFIQAHE IQWDYGPIGY DGRTGKSLRE PGSGPDKYFQ KSSSRIGGTY WKVRYEAFQD ETFQERVHQE EETHLGILGP VIRAEVGDTI QVVFYNRASQ PFSIQPHGVF YEKNSEGTVY NDGTSHPKVA KSFEKVTYYW TVPPHAGPTA QDPACLTWMY FSAADPTRDT NSGLVGPLLV CKAGALGADG KQKGVDKEFF LLFTVFDENE SWYNNANQAA GMLDSRLLSE DVEGFQDSNR MHAINGFLFS NLPRLDMCKG DTVAWHLLGL GTETDVHGVM FEGNTVQLQG MRKGAVMLFP HTFVTAIMQP DNPGIFEIYC QAGSHREEGM QAIYNVSQCS SHQDSPRQHY QASRVYYIMA EEIEWDYCPD RSWELEWHNT SEKDSYGHVF LSNKDGLLGS KYKKAVFREY TDGTFRIPRP RSGPEEHLGI LGPLIRGEVG DILTVVFKNK ASRPYSIHAH GVLESNTGGP QAAEPGEVLT YQWNIPERSG PGPSDSACVS WIYYSAVDPI KDMYSGLVGP LVICRNGILE PNGGRNDMDR EFALLFLIFD ENQSWYLKEN IATYGPQESS HVNLKDATFL ESNKMHAING KLYANLRGLT VYQGERVAWY MLAMGQDTDI HTVHFHAESF LYQNGQSYRA DVVDLFPGTF EVVEMVASNP GTWLMHCHVT DHVHAGMETI FTVLSHEEHF STMTTITKEI GKAVILRDIG GDNVKMLGMN IPIKDVEILS SALIAICVLL LLIALALGGV VWYQHRQRKL RRNRRSILDD SFKLLSLKQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Heph Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Hephaestin (HEPH)

Alternative Name: Heph (HEPH Products)

Synonyms: HEPH Protein, C130006F04Rik Protein, Cpl Protein, mKIAA0698 Protein, sla Protein, CPL Protein, hephaestin Protein, HEPH Protein, heph Protein, Heph Protein

Background: May function as a ferroxidase for ferrous (II) to ferric ion (III) conversion and may be involved in copper transport and homeostasis. Implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1.

Molecular Weight: 128.7 kDa Including tag.

UniProt: Q9Z0Z4

Pathways: Transition Metal Ion Homeostasis