Factor VIII Protein (AA 15-629) (His tag)

Catalog No. ABIN4976686

Product Details

Target: Factor VIII (F8) (Coagulation Factor VIII (F8))

Protein Type: Recombinant

Protein Characteristics: AA 15-629

Origin: Influenza C Virus

Source: Yeast

Purification tag / Conjugate: This Factor VIII protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: EKIKICLQKQ VNSSFSLHNG FGGNLYATEE KRMFELVKPK AGASVLNQST WIGFGDSRTD QSNSAFPRSL MSAKTADKFR SLSGGSLMLS MFGPPGKVDY LYQGCGKHKV FYEGVNWSPH AAIDCYRKNW TDIKLNFQKS IYELASQSHC MSLVNALDKT IPLQVTKGVA KNCNNSFLKN PALYTQEVKP LEQICGEENL AFFTLPTQFG TYECKLHLVA SCYFIYDSKE VYNKRGCGNY FQVIYDSSGK VVGGLDNRVS PYTGNSGDTP TMQCDMLQLK PGRYSVRSSP RFLLMPERSY CFDMKEKGPV TAVQSIWGKG RKSDYAVDQA CLSTPGCMLI QKQKPYIGEA DDHHGDQEMR ELLSGLDYEA RCISQSGWVN ETSPFTEEYL LPPKFGRCPL AAKEESIPKI PDGLLIPTSG TDTTVTKPKS RIFGIDDLII GLLFVAIVEA GIGGYLLGSR KESGGGVTKE SAEKGFEKIG NDIQILRSST NIAIEKLNDR ISHDEQAIRD LTLEIENARS EALLGELGII RALLVGNISI GLQESLWELA SEITNRAGDL AVEVSPGCWI IDNNICDQSC QNFIFKFNET APVPTIPPLD TKIDLQSDPF YWGSS

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: Factor VIII (F8) (Coagulation Factor VIII (F8))

Alternative Name: HEMA (F8 Products)

Synonyms: fb61d02 Protein, wu:fb61d02 Protein, Cf-8 Protein, Cf8 Protein, FVIII Protein, AHF Protein, DXS1253E Protein, F8B Protein, F8C Protein, HEMA Protein, coagulation factor VIIi Protein, coagulation factor VIII Protein, coagulation factor VIII, procoagulant component Protein, f7i Protein, F8 Protein

Background: Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the mbrane of the endocytosed virus particle with the endosomal mbrane. Low pH in endosomes induce an irreversible conformational change in HEF2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Displays a receptor-destroying activity which is a neuraminidate-O-acetyl esterase. This activity cleaves off any receptor on the cell surface, which would otherwise prevent virions release. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell .

Molecular Weight: 70.05 kDa

UniProt: P03465