IL-7 Protein
Quick Overview for IL-7 Protein (ABIN5505704)
Target
See all IL-7 (IL7) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human Interleukin-7 / IL7 protein expressed in Insect cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- Less than 0.01 ng per μg protein as determined by the LAL method.
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Biological Activity Comment
- The ED50 is 1.0 - 5 ng/ml, corresponding to a specific activity of 0.2 - 1 x 10^6 units/mg, determined by PHA activated PBL proliferation induced by IL-7 in a dose dependent manner. Under this condition the bioactivity is equivalent
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Interleukin 7 (IL7) (AA 26-177) protein
IL7 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 7 (IL7) (AA 26-154) (Active) protein (His tag)IL7 Origin: Mouse Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Interleukin 7 (IL7) (AA 26-177) (Active) protein (His tag)IL7 Origin: Human Host: Escherichia coli (E. coli) > 95 % AcA, CC Active
Interleukin 7 (IL7) (AA 26-154) protein (His tag)IL7 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 7 (IL7) (AA 26-177) protein (His-IF2DI Tag)IL7 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 7 (IL7) (Active) protein (His tag)IL7 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE Active
Interleukin 7 (IL7) (AA 26-177) protein (GST tag)IL7 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Concentration
- > 50 μg/mL
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Buffer
- 10 mM NaH2PO4, 150 mM NaCl, pH 7.2
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- IL-7 (IL7) (Interleukin 7 (IL7))
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Alternative Name
- Interleukin-7 / IL7
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Molecular Weight
- 17 kDa
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NCBI Accession
- NP_032397
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Pathways
- JAK-STAT Signaling
Target
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