VSIG8 Protein (AA 22-263) (Fc Tag,AVI tag,Biotin)

Catalog No. ABIN5526638

Product Details

Target: VSIG8 (V-Set and Immunoglobulin Domain Containing 8 (VSIG8))

Protein Type: Recombinant

Protein Characteristics: AA 22-263

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This VSIG8 protein is labelled with Fc Tag,AVI tag,Biotin.

Brand: PrecisionAvi

Sequence: AA 22-263

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 55.5 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: VSIG8 (V-Set and Immunoglobulin Domain Containing 8 (VSIG8))

Alternative Name: VSIG8 (VSIG8 Products)

Synonyms: A030011M19 Protein, EG240916 Protein, RGD1562464 Protein, zgc:110326 Protein, V-set and immunoglobulin domain containing 8 Protein, V-set and immunoglobulin domain-containing protein 8 Protein, V-set and immunoglobulin domain containing 8a Protein, Vsig8 Protein, VSIG8 Protein, LOC100403027 Protein, vsig8a Protein

Background: V-set and immunoglobulin domain containing 8 (VSIG8), also known as C1orf204, is a type I transmembrane protein of the B7 family within the Ig superfamily. VSIG8 was identified from proteomic analysis of human hair shafts. It is expressed in the hair follicle and shaft, superficial layers of the nail matrix, and superficial layers of oral epithelium.

Molecular Weight: 55.5 kDa

NCBI Accession: NP_001013683