GCK Protein (AA 1-321) (His tag)
Quick Overview for GCK Protein (AA 1-321) (His tag) (ABIN5623657)
Target
See all GCK ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-321
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Purification tag / Conjugate
- This GCK protein is labelled with His tag.
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Sequence
- 1-321 aa: MGSSHHHHHH SSGLVPRGSH MGSMTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGF RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL
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Characteristics
- Purified Recombinant Glucokinase protein (His tagged)
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Purification
- Purified
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Biological Activity Comment
- Specific activity is > 70 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 umole of Glucose to D-glucose 6-phosphate per minute in the presence of Beta-NADP at pH 9.0 at 37C.
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Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (His tag)
custom-made GCK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (His tag)custom-made GCK Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (GST tag)GCK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (His tag)GCK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Glucokinase (Hexokinase 4) (GCK) proteinGCK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied in liquid form in 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeated freezing and thawing cycles.
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- GCK (Glucokinase (Hexokinase 4) (GCK))
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Alternative Name
- Glucokinase
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Pathways
- MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process
Target
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