V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Protein

Details for Product No. ABIN5624570
Protein Name
Baculovirus infected Insect Cells
Protein Type
Western Blotting (WB)
Purification Recombinant protein corresponds to amino acids 1 to 479 of mature human AKT2, Akt isoform 2. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. This protein was co-expressed with the p110 kinase domain leading to phosphorylation of key residues T308 and S473. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
Protein Name
Gene ID 208
UniProt P31751
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
Application Notes

Application Note: AKT2 is suitable as a control for polyclonal or monoclonal anti-AKT2 in immunological assays. Akt2 recombinant protein is expected to be phosphorylated and in an active state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Western Blot Dilution: 50 ng

Restrictions For Research Use only
Format Liquid
Concentration 1.09 mg/mL

Buffer: 20 mM Tris pH 8, 300 mM NaCl with 10 % glycerol

Stabilizer: 10 % (v/v) Glycerol

Preservative Without preservative
Storage -20 °C
Storage Comment Store vial at -70° C prior to use. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Expiry Date 12 months
Image no. 1 for V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) protein (ABIN5624570) SDS-PAGE of AKT2 Human Recombinant Protein. Lane 1: AKT2, unreduced. Lane 2: prestain...
Image no. 2 for V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) protein (ABIN5624570) Western Blot of AKT2 Human Recombinant Protein Lane 1: SuperSignal MW markers. Lane2:...
Image no. 3 for V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) protein (ABIN5624570) Western Blot of Rabbit AKT Antibodies. Lane 1: NIR MW protein ladder. Lane 2: AKT1, r...