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AKT2 Protein

AKT2 Origin: Human Host: Baculovirus infected Insect Cells Recombinant WB
Catalog No. ABIN5624569
  • Target See all AKT2 Proteins
    AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
    Protein Type
    Recombinant
    Origin
    • 10
    • 3
    • 1
    • 1
    Human
    Source
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Baculovirus infected Insect Cells
    Application
    Western Blotting (WB)
    Purification
    Recombinant protein corresponds to amino acids 1 to 479 of mature human AKT2, Akt isoform 2. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
  • Application Notes

    Application Note: AKT2 (phosphatase treated) is suitable as a control for polyclonal or monoclonal anti-AKT2 in immunological assays. Akt2 recombinant protein is dephosphorylated and in an inactive state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized.

    Western Blot Dilution: 50 ng

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.17 mg/mL
    Buffer

    Buffer: 20 mM Tris pH 8, 300 mM NaCl with 10 % glycerol

    Stabilizer: 10 % (v/v) Glycerol

    Preservative
    Without preservative
    Storage
    -20 °C
    Storage Comment
    Store vial at -70° C prior to use. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    Expiry Date
    12 months
  • Target
    AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
    Alternative Name
    AKT2 (AKT2 Products)
    Synonyms
    AKT2 Protein, pkbb Protein, pkbbeta Protein, pkbss Protein, prkbb Protein, rac-beta Protein, v-akt2 Protein, 2410016A19Rik Protein, AW554154 Protein, PKB Protein, PKBbeta Protein, HIHGHH Protein, PKBB Protein, PKBBETA Protein, PRKBB Protein, RAC-BETA Protein, cb945 Protein, akt2-a Protein, AKT serine/threonine kinase 2 Protein, v-akt murine thymoma viral oncogene homolog 2 Protein, thymoma viral proto-oncogene 2 Protein, v-akt murine thymoma viral oncogene homolog 2 L homeolog Protein, AKT2 Protein, akt2 Protein, Akt2 Protein, akt2.L Protein
    Gene ID
    208
    UniProt
    P31751
    Pathways
    PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
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