AKT2 Protein (Myc-DYKDDDDK Tag)
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- Target See all AKT2 Proteins
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This AKT2 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human AKT2 / PKB beta protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- AKT2 activity verified in a biochemical assay:,AKT2 activity verified in a biochemical assay:
- Top Product
- Discover our top product AKT2 Protein
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V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) protein
AKT2 Origin: Human Host: Baculovirus infected Insect Cells Recombinant WB
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 1-123) protein (His-IF2DI Tag)AKT2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 100-189) protein (GST tag)AKT2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) protein (GST tag,His tag)AKT2 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- > 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
- Alternative Name
- Akt2,pkb beta (AKT2 Products)
- Synonyms
- AKT2 Protein, pkbb Protein, pkbbeta Protein, pkbss Protein, prkbb Protein, rac-beta Protein, v-akt2 Protein, 2410016A19Rik Protein, AW554154 Protein, PKB Protein, PKBbeta Protein, HIHGHH Protein, PKBB Protein, PKBBETA Protein, PRKBB Protein, RAC-BETA Protein, cb945 Protein, akt2-a Protein, AKT serine/threonine kinase 2 Protein, v-akt murine thymoma viral oncogene homolog 2 Protein, thymoma viral proto-oncogene 2 Protein, v-akt murine thymoma viral oncogene homolog 2 L homeolog Protein, AKT2 Protein, akt2 Protein, Akt2 Protein, akt2.L Protein
- Background
- This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.
- Molecular Weight
- 55.6 kDa
- NCBI Accession
- NP_001617
- Pathways
- PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
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