Nerve Growth Factor Protein (NGF) (AA 19-239) (His tag)
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- Target See all Nerve Growth Factor (NGF) Proteins
- Nerve Growth Factor (NGF)
- Biological Activity
- Active
- Protein Characteristics
- AA 19-239
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Nerve Growth Factor protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 97 %
- Biological Activity Comment
- Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. As reported, when the pheochromocytoma cell line PC12 is exposed to nerve growth factor (NGF), the cells respond over a period of a week by ceasing cell division and extending neurites (Greene and Tischler, 1976). The cells were grown in Ham's F12K containing 5% fetal calf serum and 10% horse serum on polylysine or collagen coated plates. When cells reached log phase growth , fresh medium was added together with 10ng/mL of NGF, then cells were observed by inverted microscope everyday. Cell division ceasing and differentiation of PC12 cells after incubation with NGF (10ng/mL) for 6 days was shown in Figure1.Control group which received no NGF displayed no neurite outgrowth and cells multiply rapidly.
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- Discover our top product NGF Protein
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Nerve Growth Factor (NGF) (Active) protein
NGF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant SDS, Func Active
Nerve Growth Factor (NGF) (Active) proteinNGF Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS, Func Active
Nerve Growth Factor (NGF) (AA 122-239) protein (His tag)NGF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Nerve Growth Factor (NGF) (AA 200-299) protein (GST tag)NGF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Nerve Growth Factor (NGF) (AA 122-241) protein (His tag)Crystallography grade NGF Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Nerve Growth Factor (NGF) (AA 122-241) protein (His tag)Crystallography grade NGF Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
- Isoelectric Point: 10.1
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- Nerve Growth Factor (NGF)
- Abstract
- NGF Products
- Synonyms
- Beta-NGF Protein, HSAN5 Protein, NGFB Protein, Ngfb Protein, ngfb Protein, ns:zft386 Protein, xx:zft386 Protein, zgc:109730 Protein, beta-NGF Protein, hsan5 Protein, beta-ngf Protein, NGF Protein, ngf Protein, nerve growth factor Protein, nerve growth factor b (beta polypeptide) Protein, nerve growth factor S homeolog Protein, NGF Protein, Ngf Protein, ngfb Protein, ngf Protein, ngf.S Protein
- Background
- Alternative Names: NGFB, Beta-NGF, HSAN5, NGF-B, Beta-Nerve Growth Factor
- Molecular Weight
- 30/33kDa
- UniProt
- P22894
- Pathways
- Regulation of Cell Size
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