FZR1 Protein (AA 1-493, full length) (His-SUMO Tag)

Catalog No. ABIN5711969

Product Details

Target: FZR1 (Fizzy/cell Division Cycle 20 Related 1 (FZR1))

Protein Type: Recombinant

Protein Characteristics: full length, AA 1-493

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This FZR1 protein is labelled with His-SUMO Tag.

Application: SDS-PAGE (SDS)

Sequence: MDQDYERRLL RQIVIQNENT MPRVTEMRRT LTPASSPVSS PSKHGDRFIP SRAGANWSVN FHRINENEKS PSQNRKAKDA TSDNGKDGLA YSALLKNELL GAGIEKVQDP QTEDRRLQPS TPEKKGLFTY SLSTKRSSPD DGNDVSPYSL SPVSNKSQKL LRSPRKPTRK ISKIPFKVLD APELQDDFYL NLVDWSSLNV LSVGLGTCVY LWSACTSQVT RLCDLSVEGD SVTSVGWSER GNLVAVGTHK GFVQIWDAAA GKKLSMLEGH TARVGALAWN AEQLSSGSRD RMILQRDIRT PPLQSERRLQ GHRQEVCGLK WSTDHQLLAS GGNDNKLLVW NHSSLSPVQQ YTEHLAAVKA IAWSPHQHGL LASGGGTADR CIRFWNTLTG QPLQCIDTGS QVCNLAWSKH ANELVSTHGY SQNQILVWKY PSLTQVAKLT GHSYRVLYLA MSPDGEAIVT GAGDETLRFW NVFSKTRSTK ESVSVLNLFT RIR

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: FZR1 (Fizzy/cell Division Cycle 20 Related 1 (FZR1))

Alternative Name: FZR (FZR1 Products)

Synonyms: CDH1-C Protein, FYR Protein, FZR Protein, CDH1 Protein, fzr1 Protein, CDC20C Protein, FZR2 Protein, HCDH Protein, HCDH1 Protein, AW108046 Protein, Cdh1 Protein, Fyr Protein, zgc:55450 Protein, fizzy and cell division cycle 20 related 1 Protein, fizzy/cell division cycle 20 related 1 Protein, fizzy-related protein homolog Protein, fizzy/cell division cycle 20 related 1 (Drosophila) Protein, fizzy/cell division cycle 20 related 1a Protein, Fzr1 Protein, CDH1-C Protein, FZR1 Protein, LOC100027495 Protein, LOC100179951 Protein, fzr1a Protein

Background: Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacent of CDC20, and activates the APC/C during anaphase and telophase. The APC/C rains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate praturely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.

Molecular Weight: 70.73 kDa

UniProt: Q9UM11

Pathways: DNA Replication, Synthesis of DNA