DNMT3A Protein (AA 680-902, partial) (His tag)
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- Target See all DNMT3A Proteins
- DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 680-902, partial
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This DNMT3A protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- KIMYVGDVRS VTQKHIQEWG PFDLVIGGSP CNDLSIVNPA RKGLYEGTGR LFFEFYRLLH DARPKEGDDR PFFWLFENVV AMGVSDKRDI SRFLESNPVM IDAKEVSAAH RARYFWGNLP GMNRPLASTV NDKLELQECL EHGRIAKFSK VRTITTRSNS IKQGKDQHFP VFMNEKEDIL WCTEMERVFG FPVHYTDVSN MSRLARQRLL GRSWSVPVIR HLF
- Purification
- SDS-PAGE
- Purity
- > 90 %
- Top Product
- Discover our top product DNMT3A Protein
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DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (Transcript Variant 3) (Active) protein (Myc-DYKDDDDK Tag)
DNMT3A Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD Active
DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 692-912) protein (His-IF2DI Tag)DNMT3A Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 803-912) protein (GST tag)DNMT3A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 1-912) protein (GST tag)DNMT3A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 1-912) protein (Strep Tag)Crystallography grade DNMT3A Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 1-908) protein (Strep Tag)Crystallography grade DNMT3A Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Storage
- -80 °C,4 °C,-20 °C
- Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- Target
- DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))
- Alternative Name
- DNM3A (DNMT3A Products)
- Synonyms
- DNMT3A Protein, DNMT3A2 Protein, M.HsaIIIA Protein, MmuIIIA Protein, dnmt3a1 Protein, dnmt6 Protein, DNA methyltransferase 3 alpha Protein, DNA methyltransferase 3A Protein, DNA (cytosine-5-)-methyltransferase 3 alpha b Protein, DNMT3A Protein, Dnmt3a Protein, dnmt3a Protein, dnmt3ab Protein
- Background
- Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.
- Molecular Weight
- 29.9 kDa
- UniProt
- Q9Y6K1
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