NCAP (AA 1-168), (Extracellular) protein (His tag)
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- Target
- NCAP
- Protein Type
- Recombinant
- Protein Characteristics
- Extracellular, AA 1-168
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Origin
- Influenza B Virus
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Source
- Yeast
- Purification tag / Conjugate
- His tag
- Application
- SDS-PAGE (SDS)
- Sequence
- MSNMDIDGIN TGTIDKTPEE IISGTSGATR PIIRPATLAP PSNKRTRNPS PERATTSSEA DVGRKTQKKQ TPTEIKKSVY NMVVKLGEFY NQMMVKAGLN DDMERNLIQN AHAVERILLA ATDDKKTEFQ KKKNARDVKE GKEEIDHNKT GGTFYKMVRD DKTIYFSP
- Purification
- SDS-PAGE
- Purity
- > 90 %
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NCAP (AA 1-391), (full length) protein (His-SUMO Tag)
Origin: Bovine Respiratory Syncitial Virus (BRSV) Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
NCAP (AA 188-739), (partial) protein (His-SUMO Tag)Origin: Zaire ebolavirus Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
NCAP (AA 1-450), (full length) protein (His tag)Origin: Rabies Virus Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
NCAP (AA 1-560), (full length) protein (His-SUMO Tag)Origin: Influenza B Virus Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
NCAP (AA 1-168), (Extracellular) protein (GST tag)Origin: Influenza B Virus Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
NCAP (AA 1-498), (full length) protein (His-SUMO Tag)Origin: Influenza A Virus H7N7 Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
NCAP (AA 1-498), (full length) protein (His tag)Origin: Influenza A Virus H3N2 Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
NCAP (AA 1-450), (full length) protein (His tag)Origin: Rabies Virus Host: Yeast Recombinant > 90 % SDS
NCAP (AA 1-498), (full length) protein (His tag)Origin: Influenza A Virus H1N1 Host: Yeast Recombinant > 90 % SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Storage
- -80 °C,4 °C,-20 °C
- Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- Target
- NCAP
- Target Type
- Viral Protein
- Background
- Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as tplate for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP ses to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus .
- Molecular Weight
- 20.8 kDa
- UniProt
- P04666
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