FBLIM1 Protein (AA 1-373) (His tag)
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- Target See all FBLIM1 Proteins
- FBLIM1 (Filamin Binding LIM Protein 1 (FBLIM1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-373
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This FBLIM1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMASKPEK RVASSVFITL APPRRDVAVA EEVRQAVCEA RRGRPWEAPA PMKTPEAGLA GRPSPWTTPG RAAATVPAAP MQLFNGGCPP PPPVLDGEDV LPDLDLLPPP PPPPPVLLPS EEEAPAPMGA SLIADLEQLH LSPPPPPPQA PAEGPSVQPG PLRPMEEELP PPPAEPVEKG ASTDICAFCH KTVSPRELAV EAMKRQYHAQ CFTCRTCRRQ LAGQSFYQKD GRPLCEPCYQ DTLERCGKCG EVVRDHIIRA LGQAFHPSCF TCVTCARCIG DESFALGSQN EVYCLDDFYR KFAPVCSICE NPIIPRDGKD AFKIECMGRN FHENCYRCED CRILLSVEPT DQGCYPLNNH LFCKPCHVKR SAAGCC
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product FBLIM1 Protein
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Filamin Binding LIM Protein 1 (FBLIM1) (AA 1-373) protein (His tag)
FBLIM1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Filamin Binding LIM Protein 1 (FBLIM1) (AA 1-200) protein (His tag)FBLIM1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Filamin Binding LIM Protein 1 (FBLIM1) (AA 270-373) protein (GST tag)FBLIM1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Filamin Binding LIM Protein 1 (FBLIM1) (AA 1-373) protein (Strep Tag)Crystallography grade FBLIM1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Filamin Binding LIM Protein 1 (FBLIM1) (AA 1-375) protein (Strep Tag)Crystallography grade FBLIM1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Filamin Binding LIM Protein 1 (FBLIM1) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)FBLIM1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Filamin Binding LIM Protein 1 (FBLIM1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)FBLIM1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- FBLIM1 (Filamin Binding LIM Protein 1 (FBLIM1))
- Alternative Name
- FBLIM1 (FBLIM1 Products)
- Synonyms
- FBLIM1 Protein, CAL Protein, FBLP-1 Protein, FBLP1 Protein, 2410043F08Rik Protein, Cal Protein, Fblp1 Protein, Gt10 Protein, migfilin Protein, migfilin(s) Protein, filamin binding LIM protein 1 Protein, filamin binding LIM protein 1 S homeolog Protein, FBLIM1 Protein, fblim1.S Protein, Fblim1 Protein
- Background
- Filamin binding LIM protein 1, also known as FBLIM1, serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. This protein is implicated in cell shape modulation (spreading) and motility. It may participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. FBLIM1 promotes activation of integrins and regulates integrin-mediated cell-cell adhesion. Recombinant human FBLIM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 43.1 kDa (396aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_060026
- UniProt
- Q8WUP2
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