IL1R2 Protein (AA 14-343) (His tag)

Catalog No. ABIN5777947

Product Details

Target: IL1R2 (Interleukin 1 Receptor, Type II (IL1R2))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 14-343

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This IL1R2 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: FTLQPAAHTG AARSCRFRGR HYKREFRLEG EPVALRCPQV PYWLWASVSP RINLTWHKND SARTVPGEEE TRMWAQDGAL WLLPALQEDS GTYVCTTRNA SYCDKMSIEL RVFENTDAFL PFISYPQILT LSTSGVLVCP DLSEFTRDKT DVKIQWYKDS LLLDKDNEKF LSVRGTTHLL VHDVALEDAG YYRCVLTFAH EGQQYNITRS IELRIKKKKE ETIPVIISPL KTISASLGSR LTIPCKVFLG TGTPLTTMLW WTANDTHIES AYPGGRVTEG PRQEYSENNE NYIEVPLIFD PVTREDLHMD FKCVVHNTLS FQTLRTTVKE LEHHHHHH

Purity: > 90 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal to 400 ng/ml with IL-1beta.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: IL1R2 (Interleukin 1 Receptor, Type II (IL1R2))

Alternative Name: IL1R2 (IL1R2 Products)

Synonyms: CD121b Protein, CDw121b Protein, IL-1R-2 Protein, IL-1RT-2 Protein, IL-1RT2 Protein, IL1R2c Protein, IL1RB Protein, Il1r-2 Protein, IL-1R2 Protein, interleukin 1 receptor type 2 Protein, interleukin 1 receptor, type II Protein, interleukin-like Protein, IL1R2 Protein, Il1r2 Protein, KK34 Protein

Background: IL1R2, also known as interleukin-1 receptor type 2 isoform 1, is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein is expressed predominantly by, T cells, fibroblasts and endothelial cells. It binds interleukin-1 alpha (IL1A), interleukin-1beta (IL1B), and interleukin 1 receptor antagonist (IL1RN), preventing them from binding to their regular receptors and thereby inhibiting the transduction of their signaling. Also, Interleukin-4 (IL4) is reported to antagonize the activity of interleukin-1 by inducing the expression and release of this cytokine. Recombinant human IL1R2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 38.8kDa (338aa) 40-57kDa (SDS-PAGE under reducing conditions)

NCBI Accession: NP_004624

UniProt: P27930

Pathways: NF-kappaB Signaling