WWTR1 Protein (AA 1-400) (His tag)

Catalog No. ABIN5778298

Product Details

Target: WWTR1 (WW Domain Containing Transcription Regulator 1 (WWTR1))

Protein Type: Recombinant

Protein Characteristics: AA 1-400

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This WWTR1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNPASAP PPLPPPGQQV IHVTQDLDTD LEALFNSVMN PKPSSWRKKI LPESFFKEPD SGSHSRQSST DSSGGHPGPR LAGGAQHVRS HSSPASLQLG TGAGAAGSPA QQHAHLRQQS YDVTDELPLP PGWEMTFTAT GQRYFLNHIE KITTWQDPRK AMNQPLNHMN LHPAVSSTPV PQRSMAVSQP NLVMNHQHQQ QMAPSTLSQQ NHPTQNPPAG LMSMPNALTT QQQQQQKLRL QRIQMERERI RMRQEELMRQ EAALCRQLPM EAETLAPVQA AVNPPTMTPD MRSITNNSSD PFLNGGPYHS REQSTDSGLG LGCYSVPTTP EDFLSNVDEM DTGENAGQTP MNINPQQTRF PDFLDCLPGT NVDLGTLESE DLIPLFNDVE SALNKSEPFL TWL

Purity: > 85 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Denatured

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.4M uREA, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: WWTR1 (WW Domain Containing Transcription Regulator 1 (WWTR1))

Alternative Name: WWTR1 (WWTR1 Products)

Synonyms: WWTR1 Protein, TAZ Protein, 2310058J06Rik Protein, 2610021I22Rik Protein, C78399 Protein, Taz Protein, WW domain containing transcription regulator 1 L homeolog Protein, WW domain containing transcription regulator 1 Protein, wwtr1.L Protein, wwtr1 Protein, WWTR1 Protein, Wwtr1 Protein

Background: WWTR1 is a transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Recombinant human WWTR1 protein, fused to His-tag at N-terminus, was expressed in E.coli .

Molecular Weight: 46.5kDa (423aa)

NCBI Accession: NP_001161752

UniProt: Q9GZV5

Pathways: Regulation of Lipid Metabolism by PPARalpha