U2AF1 Protein (AA 1-240) (His tag)
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- Target See all U2AF1 Proteins
- U2AF1 (U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-240
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This U2AF1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMAEYLAS IFGTEKDKVN CSFYFKIGAC RHGDRCSRLH NKPTFSQTIL IQNIYRNPQN SAQTADGSHC AVSDVEMQEH YDEFFEEVFT EMEEKYGEVE EMNVCDNLGD HLVGNVYVKF RREEDAEKAV IDLNNRWFNG QPIHAELSPV TDFREACCRQ YEMGECTRGG FCNFMHLKPI SRELRRELYG RRRKKHRSRS RSRERRSRSR DRGRGGGGGG GGGGGGRERD RRRSRDRERS GRF
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product U2AF1 Protein
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U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1) (AA 56-155) protein (GST tag)
U2AF1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1) (AA 1-240) protein (GST tag)U2AF1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1) (AA 2-240) protein (His tag)Crystallography grade U2AF1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1) (AA 2-239) protein (His tag)Crystallography grade U2AF1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Denatured
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.4M uREA, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- U2AF1 (U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1))
- Alternative Name
- u2AF1 (U2AF1 Products)
- Synonyms
- DDBDRAFT_0218665 Protein, DDBDRAFT_0233354 Protein, DDB_0218665 Protein, DDB_0233354 Protein, 2010107D16Rik Protein, 35kDa Protein, FP793 Protein, RN Protein, RNU2AF1 Protein, U2AF35 Protein, U2AFBP Protein, CHUNP6860 Protein, zgc:111896 Protein, U2 small nuclear RNA auxiliary factor 1 Protein, CCHC-type zinc finger-containing protein Protein, splicing factor U2AF 35 kDa subunit Protein, U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 Protein, U2 small nuclear RNA auxiliary factor 1 S homeolog Protein, U2AF1 Protein, u2af1 Protein, LOC737227 Protein, U2af1 Protein, u2af1.S Protein
- Background
- U2AF1 belongs to the splicing factor SR family of genes. u2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of u2 snRNP to the pre-mRNA branch site. This protein is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Recombinant human u2AF1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
- Molecular Weight
- 30.3 kDa (263aa)
- NCBI Accession
- NP_001020374
- UniProt
- Q01081
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