B3GAT3 Protein (AA 29-335) (His tag)
-
- Target See all B3GAT3 Proteins
- B3GAT3 (beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 29-335
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This B3GAT3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSQPCDCLP PLRAAAEQLR QKDLRISQLQ AELRRPPPAP AQPPEPEALP TIYVVTPTYA RLVQKAELVR LSQTLSLVPR LHWLLVEDAE GPTPLVSGLL AASGLLFTHL VVLTPKAQRL REGEPGWVHP RGVEQRNKAL DWLRGRGGAV GGEKDPPPPG TQGVVYFADD DNTYSRELFE EMRWTRGVSV WPVGLVGGLR FEGPQVQDGR VVGFHTAWEP SRPFPVDMAG FAVALPLLLD KPNAQFDSTA PRGHLESSLL SHLVDPKDLE PRAANCTRVL VWHTRTEKPK MKQEEQLQRQ GRGSDPAIEV
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product B3GAT3 Protein
-
beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3) (AA 1-335) protein (GST tag)
B3GAT3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3) (AA 236-335) protein (GST tag)B3GAT3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3) (AA 29-335) protein (His tag)Crystallography grade B3GAT3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3) (AA 29-335) protein (His tag)Crystallography grade B3GAT3 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3) (AA 1-335) protein (Strep Tag)Crystallography grade B3GAT3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3) (AA 1-335) protein (Strep Tag)Crystallography grade B3GAT3 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3) protein (Myc-DYKDDDDK Tag)B3GAT3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- Target
- B3GAT3 (beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3))
- Alternative Name
- B3GAT3 (B3GAT3 Products)
- Synonyms
- GLCATI Protein, 2810405M13Rik Protein, GlcUAT-I Protein, Glcat-i Protein, beta-1,3-glucuronyltransferase 3 Protein, beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) Protein, B3GAT3 Protein, B3gat3 Protein
- Background
- Beta-1,3-glucuronyltransferase 3, also known as B3GAT3, is involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. This protein can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. It shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. Recombinant human B3GAT3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 36.4 kDa (330aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_036332
- UniProt
- O94766
- Pathways
- Glycosaminoglycan Metabolic Process
-
