SEMG1 Protein (AA 24-462) (His tag)
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- Target See all SEMG1 Proteins
- SEMG1 (Semenogelin I (SEMG1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 24-462
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SEMG1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSQKGGSKG RLPSEFSQFP HGQKGQHYSG QKGKQQTESK GSFSIQYTYH VDANDHDQSR KSQQYDLNAL HKTTKSQRHL GGSQQLLHNK QEGRDHDKSK GHFHRVVIHH KGGKAHRGTQ NPSQDQGNSP SGKGISSQYS NTEERLWVHG LSKEQTSVSG AQKGRKQGGS QSSYVLQTEE LVANKQQRET KNSHQNKGHY QNVVEVREEH SSKVQTSLCP AHQDKLQHGS KDIFSTQDEL LVYNKNQHQT KNLNQDQQHG RKANKISYQS SSTEERRLHY GENGVQKDVS QSSIYSQTEE KAQGKSQKQI TIPSQEQEHS QKANKISYQS SSTEERRLHY GENGVQKDVS QRSIYSQTEK LVAGKSQIQA PNPKQEPWHG ENAKGESGQS TNREQDLLSH EQKGRHQHGS HGGLDIVIIE QEDDSDRHLA QHLNNDRNPL FT
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product SEMG1 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 7.5) containing 0.5M NaCl, 10 % glycerol, 250 mM Imidazole, 0.1 mM PMSF, 1 mM DTT.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- SEMG1 (Semenogelin I (SEMG1))
- Alternative Name
- SEMG1 (SEMG1 Products)
- Synonyms
- SEMG1 Protein, SEMG1a Protein, CT103 Protein, SEMG Protein, SGI Protein, dJ172H20.2 Protein, RATSVPIIA Protein, SVPIIA Protein, SVS2P Protein, Svp1 Protein, Svs2 Protein, Svs2p2 Protein, BB115391 Protein, Semg1 Protein, SvsII Protein, semenogelin II Protein, semenogelin I Protein, semenogelin-2 Protein, seminal vesicle secretory protein 2 Protein, SEMG2 Protein, SEMG1 Protein, LOC100408385 Protein, Semg1 Protein, Svs2 Protein
- Background
- SEMG1 is the predominant protein in semen. The secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. The prostate-specific antigen (PSA) protease processes this protein into smaller peptides, with each possibly having a separate function. The proteolysis process breaks down the gel matrix and allows the spermatozoa to move more freely. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human SEMG1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 52kDa (462aa)
- NCBI Accession
- NP_002998
- UniProt
- P04279
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