NSMCE1 Protein (AA 1-266) (His tag)
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- Target See all NSMCE1 Proteins
- NSMCE1 (Non-SMC Element 1 Homolog (NSMCE1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-266
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NSMCE1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMQGSTRR MGVMTDVHRR FLQLLMTHGV LEEWDVKRLQ THCYKVHDRN ATVDKLEDFI NNINSVLESL YIEIKRGVTE DDGRPIYALV NLATTSISKM ATDFAENELD LFRKALELII DSETGFASST NILNLVDQLK GKKMRKKEAE QVLQKFVQNK WLIEKEGEFT LHGRAILEME QYIRETYPDA VKICNICHSL LIQGQSCETC GIRMHLPCVA KYFQSNAEPR CPHCNDYWPH EIPKVFDPEK ERESGVLKSN KKSLRSRQH
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product NSMCE1 Protein
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Non-SMC Element 1 Homolog (NSMCE1) (AA 1-266) protein (His tag)
NSMCE1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Non-SMC Element 1 Homolog (NSMCE1) (AA 1-256) protein (GST tag)NSMCE1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Non-SMC Element 1 Homolog (NSMCE1) protein (Myc-DYKDDDDK Tag)NSMCE1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- NSMCE1 (Non-SMC Element 1 Homolog (NSMCE1))
- Alternative Name
- NSMCE1 (NSMCE1 Products)
- Synonyms
- zgc:92777 Protein, NSMCE1 Protein, NSE1 Protein, 2510027N19Rik Protein, RGD1307760 Protein, NSE1 homolog, SMC5-SMC6 complex component Protein, NSE1 homolog, SMC5-SMC6 complex component L homeolog Protein, nsmce1 Protein, NSMCE1 Protein, nsmce1.L Protein, Nsmce1 Protein
- Background
- NSMCE1 is a component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). NSMCE1 has in vitro ubiquitin ligase activity in presence of NDNL2. Recombinant human NSMCE1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 33 kDa (289aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_659547
- UniProt
- Q8WV22
- Pathways
- Positive Regulation of Response to DNA Damage Stimulus
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