RPL35A Protein (AA 1-110) (His tag)

Catalog No. ABIN5853250

Product Details

Target: RPL35A (Ribosomal Protein L35a (RPL35A))

Protein Type: Recombinant

Protein Characteristics: AA 1-110

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This RPL35A protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSGRLWS KAIFAGYKRG LRNQREHTAL LKIEGVYARD ETEFYLGKRC AYVYKAKNNT VTPGGKPNKT RVIWGKVTRA HGNSGMVRAK FRSNLPAKAI GHRIRVMLYP SRI

Purity: > 85 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Denatured

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.4M urea, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: RPL35A (Ribosomal Protein L35a (RPL35A))

Alternative Name: RPL35A (RPL35A Products)

Synonyms: DBA5 Protein, L35A Protein, 2810431L15Rik Protein, Rpl35 Protein, ribosomal protein L35a Protein, ribosomal protein L35A Protein, ribosomal protein L35a L homeolog Protein, RPL35A Protein, Rpl35a Protein, rpl35a.L Protein

Background: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL35A is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L35AE family of ribosomal proteins. It is located in the cytoplasm. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome. Although this gene was originally mapped to chromosome 18, it has been established that it is located at 3q29-qter. Transcript variants utilizing alternative transcription initiation sites and alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Recombinant human RPL35A protein, fused to His-tag at N-terminus, was expressed in E.coli.

Molecular Weight: 14.9 kDa (133aa)

NCBI Accession: NP_000987

UniProt: P18077