DBR1 Protein (AA 228-544) (His tag)
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- Target See all DBR1 Proteins
- DBR1 (Debranching Enzyme Homolog 1 (DBR1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 228-544
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This DBR1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSHVKFAAL MQHQAKDKGQ TARATKFLAL DKCLPHRDFL QILEIEHDPS APDYLEYDIE WLTILRATDD LINVTGRLWN MPENNGLHAR WDYSATEEGM KEVLEKLNHD LKVPCNFSVT AACYDPSKPQ TQMQLIHRIN PQTTEFCAQL GIIDINVRLQ KSKEEHHVCG EYEEQDDVES NDSGEDQSEY NTDTSALSSI NPDEIMLDEE EDEDSIVSAH SGMNTPSVEP SDQASEFSAS FSDVRILPGS MIVSSDDTVD STIDREGKPG GTVESGNGED LTKVPLKRLS DEHEPEQRKK IKRRNQAIYA AVDDDDDDAA
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product DBR1 Protein
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Debranching Enzyme Homolog 1 (DBR1) (AA 1-544) protein (GST tag)
DBR1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Debranching Enzyme Homolog 1 (DBR1) (AA 445-538) protein (GST tag)DBR1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Debranching Enzyme Homolog 1 (DBR1) (AA 1-544) protein (Strep Tag)Crystallography grade DBR1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Debranching Enzyme Homolog 1 (DBR1) (AA 1-550) protein (Strep Tag)Crystallography grade DBR1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Debranching Enzyme Homolog 1 (DBR1) protein (Myc-DYKDDDDK Tag)DBR1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- DBR1 (Debranching Enzyme Homolog 1 (DBR1))
- Alternative Name
- DBR1 (DBR1 Products)
- Synonyms
- AW018415 Protein, wu:fc08f08 Protein, zgc:63930 Protein, ARABIDOPSIS THALIANA DEBRANCHING ENZYME 1 Protein, ATDBR1 Protein, F28M20.40 Protein, F28M20_40 Protein, debranching enzyme 1 Protein, dbr1 Protein, dbr1-a Protein, dbr1-b Protein, debranching RNA lariats 1 Protein, debranching enzyme homolog 1 (S. cerevisiae) Protein, Lariat debranching enzyme Protein, debranching enzyme 1 Protein, RNA lariat debranching enzyme Dbr1 Protein, debranching RNA lariats 1 S homeolog Protein, debranching RNA lariats 1 L homeolog Protein, DBR1 Protein, Dbr1 Protein, dbr1 Protein, dbr-1 Protein, dbr1.S Protein, dbr1.L Protein
- Background
- DBR1 is an RNA lariat debranching enzyme that hydrolyzes 2'-5' prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication. Recombinant human DBR1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 38 kDa (340aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
- NCBI Accession
- NP_057300
- UniProt
- Q9UK59
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