DNALI1 Protein (AA 1-280) (His tag)
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- Target See all DNALI1 Proteins
- DNALI1 (Dynein, Axonemal, Light Intermediate Chain 1 (DNALI1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-280
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This DNALI1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMVTANKA HTGQGSCWVA TLASAMIPPA DSLLKYDTPV LVSRNTEKRS PKARLLKVSP QQPGPSGSAP QPPKTKLPST PCVPDPTKQA EEILNAILPP REWVEDTQLW IQQVSSTPST RMDVVHLQEQ LDLKLQQRQA RETGICPVRR ELYSQCFDEL IREVTINCAE RGLLLLRVRD EIRMTIAAYQ TLYESSVAFG MRKALQAEQG KSDMERKIAE LETEKRDLER QVNEQKAKCE ATEKRESERR QVEEKKHNEE IQFLKRTNQQ LKAQLEGIIA PKK
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product DNALI1 Protein
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Dynein, Axonemal, Light Intermediate Chain 1 (DNALI1) (AA 1-258) protein (His tag)
DNALI1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Dynein, Axonemal, Light Intermediate Chain 1 (DNALI1) (AA 1-258) protein (GST tag)DNALI1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Dynein, Axonemal, Light Intermediate Chain 1 (DNALI1) (AA 1-258) protein (Strep Tag)Crystallography grade DNALI1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Dynein, Axonemal, Light Intermediate Chain 1 (DNALI1) (AA 1-258) protein (Strep Tag)Crystallography grade DNALI1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Dynein, Axonemal, Light Intermediate Chain 1 (DNALI1) protein (Myc-DYKDDDDK Tag)DNALI1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT, 0.1 mM PMSF
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- DNALI1 (Dynein, Axonemal, Light Intermediate Chain 1 (DNALI1))
- Alternative Name
- DNALI1 (DNALI1 Products)
- Synonyms
- zgc:171493 Protein, zgc:171595 Protein, P28 Protein, dJ423B22.5 Protein, hp28 Protein, 1700023A09Rik Protein, AW049135 Protein, dynein axonemal light intermediate chain 1 Protein, dynein, axonemal, light intermediate chain 1 Protein, dynein axonemal light intermediate chain 1 S homeolog Protein, dynein, axonemal, light intermediate polypeptide 1 Protein, DNALI1 Protein, dnali1 Protein, dnali1.S Protein, Dnali1 Protein
- Background
- DNALI1 is the human homolog of the Chlamydomonas inner dynein arm gene, p28. The precise function of this gene is not known, however, it is a potential candidate for immotile cilia yndrome (ICS). ultrastructural defects of the inner dynein arms are seen in patients with ICS. Immotile mutant strains of Chlamydomonas, a biflagellated algae, exhibit similar defects. Recombinant human DNALI1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 34.2 kDa (303aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_003453
- UniProt
- O14645
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